HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: ARHG7 (Q14155)
Rho guanine nucleotide exchange factor 7 (Beta-Pix) (COOL-1) (PAK-interacting exchange factor beta) (p85)
Gene: ARHGEF7

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.81	1.00	0.903

Property	Details
Experiments	26186194, 28514442, 33961781, 35271311
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	SH2~SH3_2, PH~SH3_1, RhoGEF~SH3_1, SH3_1~SH3_2, PH~SH3_9, RhoGEF~SH3_9, SH3_2~SH3_9
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0007165 - signal transduction GO:0030032 - lamellipodium assembly GO:0048013 - ephrin receptor signaling pathway
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 380 Hs			1 803
+	Hs
		557687 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ARHG7_HUMAN (1-803): 0: 654: 100.00
+	Hs
		209055 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		ARHG6_HUMAN (1-802): 1.77e-161: 489: 51.48
+	Hs
		557698 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ARHG6_HUMAN (1-802): 1.77e-161: 489: 51.48
+	Hs
		557763 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ARHG2_HUMAN (62-801): 1.23e-80: 280: 13.33
+	Hs
		548645 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		VAV_HUMAN (1-778): 3.84e-111: 360: 16.96
+	Hs
		655500 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		VAV3_HUMAN (1-731): 3.11e-110: 358: 16.73
+	Hs
		411981 FYN_HUMAN (7-137): 2.1e-23: 103: 20.30		VAV_HUMAN (1-778): 3.84e-111: 360: 16.96
+	Hs
		412098 FYN_HUMAN (7-137): 2.1e-23: 103: 20.30		VAV2_HUMAN (1-712): 4.73e-111: 360: 17.04
+	Hs
		411580 LCK_HUMAN (3-145): 6.3e-19: 90.2: 21.53		VAV_HUMAN (1-778): 3.84e-111: 360: 16.96
+	Hs
		279897 ITSN2_HUMAN (3-309): 6.32e-28: 117: 18.31		TRIO_HUMAN (16-803): 4.74e-74: 266: 13.40
+	Hs
		655405 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		FGD2_HUMAN (230-629): 5.88e-78: 266: 18.64
+	Hs
		1078876 ITSN1_HUMAN (6-70): 3.77e-19: 91.7: 29.23		PREX1_HUMAN (224-582): 1.43e-76: 273: 20.66
+	Hs
		279830 LCK_HUMAN (3-145): 6.3e-19: 90.2: 21.53		TRIO_HUMAN (16-803): 4.74e-74: 266: 13.40
+	Hs
		224699 FYN_HUMAN (7-137): 2.1e-23: 103: 20.30		KALRN_HUMAN (34-659): 7.34e-70: 253: 13.25
+	Hs
		224698 LCK_HUMAN (3-145): 6.3e-19: 90.2: 21.53		KALRN_HUMAN (34-659): 7.34e-70: 253: 13.25
+	Hs
		1349275 ITSN2_HUMAN (3-309): 6.32e-28: 117: 18.31		ITSN2_HUMAN (41-259): 5.37e-11: 68.6: 18.55
+	Hs
		1078919 ITSN2_HUMAN (3-309): 6.32e-28: 117: 18.31		ITSN1_HUMAN (21-245): 9.17e-09: 61.3: 14.68
+	Hs
		655386 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		ITSN1_HUMAN (21-245): 9.17e-09: 61.3: 14.68
+	Hs
		1078828 ITSN1_HUMAN (6-70): 3.77e-19: 91.7: 29.23		ITSN1_HUMAN (21-245): 9.17e-09: 61.3: 14.68