HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: GIT2 (Q14161)
ARF GTPase-activating protein GIT2 (ARF GAP GIT2) (Cool-interacting tyrosine-phosphorylated protein 2) (CAT-2) (CAT2) (G protein-coupled receptor kinase-interactor 2) (GRK-interacting protein 2)
Gene: GIT2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.74	0.80	0.770

Property	Details
Experiments	26186194, 28514442, 33961781
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	Ank_2~SH3_1, Ank_2~SH3_9
Common GO terms	-
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 380 Hs			1 759
+	Hs
		557688 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		GIT2_HUMAN (1-759): 5.12e-178: 530: 100.00
+	Hs
		209194 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		GIT1_HUMAN (1-758): 7.22e-141: 434: 63.74
+	Hs
		557838 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		GIT1_HUMAN (1-758): 7.22e-141: 434: 63.74
+	Hs
		209187 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		ASAP1_HUMAN (3-656): 2.66e-63: 231: 15.57
+	Hs
		557823 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ASAP1_HUMAN (3-656): 2.66e-63: 231: 15.57
+	Hs
		195770 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		ASAP2_HUMAN (3-228): 8.33e-55: 205: 24.72
+	Hs
		557696 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ACAP1_HUMAN (3-295): 4.06e-61: 221: 23.39
+	Hs
		209145 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		ARAP1_HUMAN (8-186): 3.24e-49: 190: 30.22
+	Hs
		195783 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ASAP2_HUMAN (3-228): 8.33e-55: 205: 24.72
+	Hs
		557775 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ARAP1_HUMAN (8-186): 3.24e-49: 190: 30.22
+	Hs
		557660 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ANK2_HUMAN (108-437): 3.9e-45: 177: 13.13
+	Hs
		655502 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		ASAP1_HUMAN (3-656): 2.66e-63: 231: 15.57
+	Hs
		195787 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		ASAP2_HUMAN (3-228): 8.33e-55: 205: 24.72
+	Hs
		655450 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		AGAP2_HUMAN (3-224): 2.94e-54: 204: 32.00
+	Hs
		161311 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		MYPT1_HUMAN (94-670): 7.76e-54: 203: 10.75
+	Hs
		195776 FYN_HUMAN (7-137): 2.1e-23: 103: 20.30		ASAP2_HUMAN (3-228): 8.33e-55: 205: 24.72
+	Hs
		161288 FYN_HUMAN (7-137): 2.1e-23: 103: 20.30		MYPT1_HUMAN (94-670): 7.76e-54: 203: 10.75
+	Hs
		412284 FYN_HUMAN (7-137): 2.1e-23: 103: 20.30		ARAP2_HUMAN (7-186): 2.94e-51: 196: 32.24
+	Mm
		103593 P85A_MOUSE (166-379): 4.24e-24: 106: 16.36		ANK3_MOUSE (110-437): 1.02e-49: 191: 13.03
+	Hs
		257713 ITSN2_HUMAN (3-309): 6.32e-28: 117: 18.31		ANR17_HUMAN (41-254): 6.34e-28: 123: 16.28