HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: ARHG6 (Q15052)
Rho guanine nucleotide exchange factor 6 (Alpha-Pix) (COOL-2) (PAK-interacting exchange factor alpha) (Rac/Cdc42 guanine nucleotide exchange factor 6)
Gene: ARHGEF6

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.74	1.00	0.861

Property	Details
Experiments	26186194, 28514442, 33961781
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	SH2~SH3_2, PH~SH3_1, RhoGEF~SH3_1, SH3_1~SH3_2, PH~SH3_9, RhoGEF~SH3_9, SH3_2~SH3_9
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0030032 - lamellipodium assembly
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 380 Hs			1 776
+	Hs
		557698 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ARHG6_HUMAN (1-776): 0: 621: 100.00
+	Hs
		209051 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		ARHG7_HUMAN (1-719): 4.57e-168: 506: 54.22
+	Hs
		557687 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ARHG7_HUMAN (1-719): 4.57e-168: 506: 54.22
+	Hs
		655500 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		VAV3_HUMAN (1-746): 7.26e-127: 400: 16.03
+	Hs
		548645 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		VAV_HUMAN (1-746): 1.59e-120: 384: 16.20
+	Hs
		411981 FYN_HUMAN (7-137): 2.1e-23: 103: 20.30		VAV_HUMAN (1-746): 1.59e-120: 384: 16.20
+	Hs
		411580 LCK_HUMAN (3-145): 6.3e-19: 90.2: 21.53		VAV_HUMAN (1-746): 1.59e-120: 384: 16.20
+	Hs
		412098 FYN_HUMAN (7-137): 2.1e-23: 103: 20.30		VAV2_HUMAN (1-746): 1.37e-111: 361: 15.14
+	Hs
		1078919 ITSN2_HUMAN (3-309): 6.32e-28: 117: 18.31		ITSN1_HUMAN (18-575): 4.42e-86: 300: 15.88
+	Hs
		1349275 ITSN2_HUMAN (3-309): 6.32e-28: 117: 18.31		ITSN2_HUMAN (17-575): 4.43e-83: 291: 16.67
+	Hs
		655386 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		ITSN1_HUMAN (18-575): 4.42e-86: 300: 15.88
+	Hs
		1078828 ITSN1_HUMAN (6-70): 3.77e-19: 91.7: 29.23		ITSN1_HUMAN (18-575): 4.42e-86: 300: 15.88
+	Hs
		655405 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		FGD2_HUMAN (200-771): 4.22e-82: 277: 14.38
+	Hs
		1078876 ITSN1_HUMAN (6-70): 3.77e-19: 91.7: 29.23		PREX1_HUMAN (218-552): 8.7e-83: 291: 23.40
+	Hs
		279897 ITSN2_HUMAN (3-309): 6.32e-28: 117: 18.31		TRIO_HUMAN (230-567): 1.42e-72: 261: 20.46
+	Hs
		411673 LCK_HUMAN (3-145): 6.3e-19: 90.2: 21.53		FGD1_HUMAN (239-698): 2.56e-80: 279: 18.33
+	Hs
		224699 FYN_HUMAN (7-137): 2.1e-23: 103: 20.30		KALRN_HUMAN (203-772): 1.73e-74: 266: 17.83
+	Hs
		224698 LCK_HUMAN (3-145): 6.3e-19: 90.2: 21.53		KALRN_HUMAN (203-772): 1.73e-74: 266: 17.83
+	Hs
		279830 LCK_HUMAN (3-145): 6.3e-19: 90.2: 21.53		TRIO_HUMAN (230-567): 1.42e-72: 261: 20.46