HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: ERBB4 (Q15303)
Receptor tyrosine-protein kinase erbB-4 (EC 2.7.10.1) (Proto-oncogene-like protein c-ErbB-4) (Tyrosine kinase-type cell surface receptor HER4) (p180erbB4) [Cleaved into: ERBB4 intracellular domain (4ICD) (E4ICD) (s80HER4)]
Gene: ERBB4

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.58	1.00	0.768

Property	Details
Experiments	22973453, 16729043
Identification Methods	pull down
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	PK_Tyr_Ser-Thr~SH2, PK_Tyr_Ser-Thr~SH3_1, PK_Tyr_Ser-Thr~SH3_9
Common GO terms	GO:0005829 - cytosol GO:0007165 - signal transduction GO:0008285 - negative regulation of cell population proliferation GO:0016477 - cell migration
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 380 Hs			1 1308
+	Hs
		557701 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ERBB4_HUMAN (1-1308): 0: 1406: 100.00
+	Hs
		208992 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		ERBB3_HUMAN (1-1258): 0: 1094: 47.33
+	Hs
		208972 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		ERBB2_HUMAN (25-1272): 0: 1090: 46.29
+	Hs
		557604 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ERBB3_HUMAN (1-1258): 0: 1094: 47.33
+	Hs
		383587 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ERBB2_HUMAN (25-1272): 0: 1090: 46.29
+	Hs
		208970 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		EGFR_HUMAN (20-1286): 0: 1039: 48.70
+	Hs
		337883 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		EGFR_HUMAN (20-1286): 0: 1039: 48.70
+	Hs
		338069 CSK_HUMAN (191-378): 3.44e-32: 127: 24.87		EGFR_HUMAN (20-1286): 0: 1039: 48.70
+	Mm
		337637 NCK1_MOUSE (1-379): 6.79e-91: 279: 68.24		ABL1_MOUSE (693-1305): 6.8e-128: 424: 24.32
+	Hs
		208996 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		EPHA2_HUMAN (671-1010): 7.32e-119: 396: 35.41
+	Hs
		337337 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ABL1_HUMAN (693-1305): 3.03e-121: 406: 25.00
+	Hs
		557644 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		EPHB1_HUMAN (624-1002): 2.47e-122: 406: 31.65
+	Hs
		209036 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		FAK1_HUMAN (703-997): 6.55e-59: 223: 39.53
+	Hs
		209001 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		ABL2_HUMAN (713-980): 1.37e-55: 214: 42.59
+	Hs
		209039 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		ACK1_HUMAN (697-970): 1.51e-54: 209: 40.43
+	Hs
		557666 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		FAK1_HUMAN (703-997): 6.55e-59: 223: 39.53
+	Hs
		557630 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ABL2_HUMAN (713-980): 1.37e-55: 214: 42.59
+	Hs
		557670 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ACK1_HUMAN (697-970): 1.51e-54: 209: 40.43
+	Rn
		88904 D4A3M8_RAT (1-380): 3.21e-98: 298: 95.79		INSR_RAT (353-660): 1.09e-31: 137: 19.05
+	Hs
		435526 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		IGF1R_HUMAN (333-691): 3.78e-32: 138: 18.11