HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: CSKI2 (Q8WXE0)
Caskin-2 (CASK-interacting protein 2)
Gene: CASKIN2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.74	1.00	0.861

Property	Details
Experiments	26186194, 28514442, 33961781
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	SH2~SH3_2, Ank_2~SH3_1, SH3_1~SH3_2, Ank_2~SH3_9, SH3_2~SH3_9
Common GO terms	GO:0005737 - cytoplasm
Homologous interactions	18

Homologous interactions

Color coding for hit scores

	1 380 Hs			1 1202
+	Hs
		557753 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		CSKI2_HUMAN (1-1202): 1.75e-114: 387: 100.00
+	Hs
		209098 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		ANS1B_HUMAN (1-610): 1.01e-79: 288: 23.45
+	Hs
		411806 LCK_HUMAN (3-145): 6.3e-19: 90.2: 21.53		CSKI2_HUMAN (1-1202): 1.75e-114: 387: 100.00
+	Hs
		1263386 SH3K1_HUMAN (8-109): 4.25e-16: 82: 16.35		CSKI2_HUMAN (1-1202): 1.75e-114: 387: 100.00
+	Mm
		1206702 ITSN1_MOUSE (6-70): 1.15e-18: 90.1: 29.23		ANS1B_MOUSE (1-610): 6.14e-79: 286: 23.75
+	Hs
		411885 LCK_HUMAN (3-145): 6.3e-19: 90.2: 21.53		KDIS_HUMAN (1-302): 1.57e-71: 265: 22.91
+	Mm
		103593 P85A_MOUSE (166-379): 4.24e-24: 106: 16.36		ANK3_MOUSE (3-442): 9.56e-64: 240: 21.54
+	Hs
		557660 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ANK2_HUMAN (315-1185): 2.96e-09: 63.9: 11.00
+	Hs
		655466 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		Q9H4D1_HUMAN (1-177): 1.88e-46: 181: 27.68
+	Ce
		220231 SRC2_CAEEL (1-202): 6.89e-21: 96: 16.75		Q93203_CAEEL (4-289): 7.47e-26: 117: 29.55
+	Hs
		1078859 ITSN1_HUMAN (6-70): 3.77e-19: 91.7: 29.23		MIB1_HUMAN (2-155): 2.17e-26: 119: 22.70
+	Ce
		666223 SEM5_CAEEL (204-364): 4.9e-16: 78.6: 30.68		Q93203_CAEEL (4-289): 7.47e-26: 117: 29.55
+	Ce
		1274646 Q9U2T9_CAEEL (51-302): 2.69e-14: 76.6: 18.40		Q93203_CAEEL (4-289): 7.47e-26: 117: 29.55
+	Hs
		655290 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		RIPK4_HUMAN (15-271): 2.22e-15: 83.6: 30.50
+	Hs
		257713 ITSN2_HUMAN (3-309): 6.32e-28: 117: 18.31		ANR17_HUMAN (2-104): 2.22e-10: 67.6: 28.85
+	Hs
		1268487 SH3K1_HUMAN (8-109): 4.25e-16: 82: 16.35		ANS1A_HUMAN (295-740): 2.77e-14: 80.3: 22.20
+	Hs
		411697 LCK_HUMAN (3-145): 6.3e-19: 90.2: 21.53		ANK3_HUMAN (56-264): 1.05e-09: 65.5: 28.23
+	Hs
		924484 GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81		ANK2_HUMAN (315-1185): 2.96e-09: 63.9: 11.00