HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: SHD (Q96IW2)
SH2 domain-containing adapter protein D
Gene: SHD

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.61	1.00	0.783

Property	Details
Experiments	32296183, unassigned1304
Identification Methods	two hybrid, two hybrid array, validated two hybrid, two hybrid prey pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	SH2~SH2, SH2~SH3_1
Common GO terms	GO:0001784 - phosphotyrosine residue binding
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 380 Hs			1 340
+	Hs
		208958 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		NCK2_HUMAN (4-335): 5.61e-55: 185: 15.45
+	Hs
		209059 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		SHB_HUMAN (56-339): 2.52e-53: 184: 41.09
+	Hs
		208989 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		NCK1_HUMAN (4-336): 5.26e-53: 180: 16.58
+	Hs
		411986 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		FYN_HUMAN (168-340): 2.04e-51: 180: 20.56
+	Hs
		557594 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		NCK1_HUMAN (4-336): 5.26e-53: 180: 16.58
+	Mm
		763403 NCK1_MOUSE (1-379): 6.79e-91: 279: 68.24		FYN_MOUSE (168-340): 2.04e-51: 180: 20.56
+	Hs
		209001 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		ABL2_HUMAN (152-337): 2.96e-41: 155: 26.18
+	Hs
		209168 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		SRMS_HUMAN (150-340): 6.34e-38: 142: 23.15
+	Hs
		557630 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ABL2_HUMAN (152-337): 2.96e-41: 155: 26.18
+	Hs
		557655 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		GRB2_HUMAN (181-338): 1.9e-44: 153: 22.01
+	Hs
		337337 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ABL1_HUMAN (152-339): 2.1e-39: 150: 24.23
+	Mm
		337637 NCK1_MOUSE (1-379): 6.79e-91: 279: 68.24		ABL1_MOUSE (152-339): 2.65e-39: 149: 24.23
+	Hs
		522833 CSK_HUMAN (191-378): 3.44e-32: 127: 24.87		SRC_HUMAN (93-340): 1.58e-54: 188: 17.51
+	Hs
		412066 CSK_HUMAN (191-378): 3.44e-32: 127: 24.87		FYN_HUMAN (168-340): 2.04e-51: 180: 20.56
+	Hs
		411626 CSK_HUMAN (191-378): 3.44e-32: 127: 24.87		LCK_HUMAN (117-340): 5.02e-50: 175: 22.13
+	Hs
		782751 CSK_HUMAN (191-378): 3.44e-32: 127: 24.87		FRK_HUMAN (126-340): 1.2e-48: 172: 20.00
+	Hs
		782767 CSK_HUMAN (191-378): 3.44e-32: 127: 24.87		GRB2_HUMAN (181-338): 1.9e-44: 153: 22.01
+	Mm
		411918 P85A_MOUSE (166-379): 4.24e-24: 106: 16.36		LCK_MOUSE (119-340): 2.98e-47: 168: 20.60
+	Hs
		337466 GRAP_HUMAN (5-169): 7.3e-28: 110: 15.74		ABL1_HUMAN (152-339): 2.1e-39: 150: 24.23