HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: ARAP1 (Q96P48)
Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 (Centaurin-delta-2) (Cnt-d2)
Gene: ARAP1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.74	1.00	0.861

Property	Details
Experiments	26186194, 28514442, 33961781
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	PH~SH3_1, PH~SH3_9
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0007015 - actin filament organization GO:0007165 - signal transduction
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 380 Hs			1 1450
+	Hs
		557775 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ARAP1_HUMAN (1-1450): 0: 1074: 100.00
+	Hs
		412284 FYN_HUMAN (7-137): 2.1e-23: 103: 20.30		ARAP2_HUMAN (290-1448): 0: 782: 39.53
+	Hs
		64627 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		RHG32_HUMAN (647-1331): 2.29e-86: 314: 16.71
+	Hs
		195770 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		ASAP2_HUMAN (322-766): 7.55e-83: 296: 19.59
+	Hs
		64651 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		RHG32_HUMAN (647-1331): 2.29e-86: 314: 16.71
+	Hs
		195783 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ASAP2_HUMAN (322-766): 7.55e-83: 296: 19.59
+	Hs
		208987 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		CHIN_HUMAN (845-1139): 1.19e-70: 247: 22.37
+	Hs
		551038 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		CHIN_HUMAN (845-1139): 1.19e-70: 247: 22.37
+	Hs
		64658 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		RHG32_HUMAN (647-1331): 2.29e-86: 314: 16.71
+	Hs
		64642 FYN_HUMAN (7-137): 2.1e-23: 103: 20.30		RHG32_HUMAN (647-1331): 2.29e-86: 314: 16.71
+	Hs
		655450 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		AGAP2_HUMAN (277-760): 7.44e-84: 302: 18.32
+	Hs
		64641 LCK_HUMAN (3-145): 6.3e-19: 90.2: 21.53		RHG32_HUMAN (647-1331): 2.29e-86: 314: 16.71
+	Hs
		195787 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		ASAP2_HUMAN (322-766): 7.55e-83: 296: 19.59
+	Hs
		195776 FYN_HUMAN (7-137): 2.1e-23: 103: 20.30		ASAP2_HUMAN (322-766): 7.55e-83: 296: 19.59
+	Hs
		557714 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		RHG21_HUMAN (84-499): 2.61e-25: 116: 10.45
+	Hs
		195795 ITSN1_HUMAN (6-70): 3.77e-19: 91.7: 29.23		ASAP2_HUMAN (322-766): 7.55e-83: 296: 19.59
+	Mm
		403434 SRC_MOUSE (6-153): 5.44e-20: 93.3: 17.33		AGAP2_MOUSE (277-760): 5.12e-81: 293: 17.25
+	Hs
		1078833 ITSN1_HUMAN (6-70): 3.77e-19: 91.7: 29.23		RHG31_HUMAN (938-1422): 2.55e-74: 275: 17.72
+	Hs
		411755 LCK_HUMAN (3-145): 6.3e-19: 90.2: 21.53		RHG21_HUMAN (84-499): 2.61e-25: 116: 10.45
+	Ce
		220207 SRC2_CAEEL (1-202): 6.89e-21: 96: 16.75		TG325_CAEEL (948-1116): 5.07e-18: 92.4: 37.06