HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: SPN90 (Q9NZQ3)
NCK-interacting protein with SH3 domain (54 kDa VacA-interacting protein) (54 kDa vimentin-interacting protein) (VIP54) (90 kDa SH3 protein interacting with Nck) (AF3p21) (Dia-interacting protein 1) (DIP-1) (Diaphanous protein-interacting protein) (SH3 adapter protein SPIN90) (WASP-interacting SH3-domain protein) (WISH) (Wiskott-Aldrich syndrome protein-interacting protein)
Gene: NCKIPSD

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.86	1.00	0.933

Property	Details
Experiments	16189514, 32296183, 28514442, 33961781, unassigned1304
Identification Methods	two hybrid, affinity chromatography technology, anti tag coimmunoprecipitation, two hybrid prey pooling approach, two hybrid array, validated two hybrid, two hybrid pooling approach
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	SH2~SH3_1, SH3_1~SH3_1
Common GO terms	GO:0005829 - cytosol
Homologous interactions	5

Homologous interactions

Color coding for hit scores

	1 380 Hs			1 722
+	Hs
		557810 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		SPN90_HUMAN (1-722): 0: 1137: 100.00
+	Hs
		1349276 ITSN2_HUMAN (3-309): 6.32e-28: 117: 18.31		SPN90_HUMAN (1-722): 0: 1137: 100.00
+	Hs
		412383 FYN_HUMAN (7-137): 2.1e-23: 103: 20.30		SPN90_HUMAN (1-722): 0: 1137: 100.00
+	Hs
		1078920 ITSN1_HUMAN (6-70): 3.77e-19: 91.7: 29.23		SPN90_HUMAN (1-722): 0: 1137: 100.00
+	Hs
		924910 GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81		SPN90_HUMAN (1-722): 0: 1137: 100.00