HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: GIT1 (Q9Y2X7)
ARF GTPase-activating protein GIT1 (ARF GAP GIT1) (Cool-associated and tyrosine-phosphorylated protein 1) (CAT-1) (CAT1) (G protein-coupled receptor kinase-interactor 1) (GRK-interacting protein 1) (p95-APP1)
Gene: GIT1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.74	1.00	0.861

Property	Details
Experiments	26186194, 28514442, 33961781
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	Ank_2~SH3_1, Ank_2~SH3_9
Common GO terms	GO:0005829 - cytosol GO:0014069 - postsynaptic density GO:0044877 - protein-containing complex binding GO:0048013 - ephrin receptor signaling pathway
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 380 Hs			1 761
+	Hs
		557838 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		GIT1_HUMAN (1-761): 1.3e-176: 527: 100.00
+	Hs
		209052 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		GIT2_HUMAN (1-758): 2.93e-147: 451: 64.39
+	Hs
		557688 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		GIT2_HUMAN (1-758): 2.93e-147: 451: 64.39
+	Hs
		209187 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		ASAP1_HUMAN (2-606): 1.67e-61: 226: 17.06
+	Hs
		557823 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ASAP1_HUMAN (2-606): 1.67e-61: 226: 17.06
+	Hs
		195770 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		ASAP2_HUMAN (2-506): 1.34e-50: 193: 15.20
+	Hs
		209145 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		ARAP1_HUMAN (8-205): 6.26e-49: 188: 29.35
+	Hs
		557696 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ACAP1_HUMAN (2-297): 2.23e-56: 208: 23.77
+	Hs
		195783 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ASAP2_HUMAN (2-506): 1.34e-50: 193: 15.20
+	Hs
		557775 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ARAP1_HUMAN (8-205): 6.26e-49: 188: 29.35
+	Hs
		557660 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ANK2_HUMAN (162-494): 7.25e-30: 129: 9.52
+	Hs
		655502 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		ASAP1_HUMAN (2-606): 1.67e-61: 226: 17.06
+	Hs
		412404 FYN_HUMAN (7-137): 2.1e-23: 103: 20.30		ASAP1_HUMAN (2-606): 1.67e-61: 226: 17.06
+	Hs
		442894 HCK_HUMAN (2-145): 5.1e-23: 102: 24.83		ASAP1_HUMAN (2-606): 1.67e-61: 226: 17.06
+	Hs
		655450 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		AGAP2_HUMAN (2-223): 3.67e-52: 198: 32.00
+	Hs
		195787 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		ASAP2_HUMAN (2-506): 1.34e-50: 193: 15.20
+	Hs
		412284 FYN_HUMAN (7-137): 2.1e-23: 103: 20.30		ARAP2_HUMAN (7-223): 6.18e-51: 195: 27.15
+	Hs
		195776 FYN_HUMAN (7-137): 2.1e-23: 103: 20.30		ASAP2_HUMAN (2-506): 1.34e-50: 193: 15.20
+	Mm
		403434 SRC_MOUSE (6-153): 5.44e-20: 93.3: 17.33		AGAP2_MOUSE (2-223): 9.71e-49: 188: 29.28
+	Mm
		103593 P85A_MOUSE (166-379): 4.24e-24: 106: 16.36		ANK3_MOUSE (237-740): 1.36e-20: 99.9: 9.25