HitPredict

Interactor 1: ACK1 (O54967)
Activated CDC42 kinase 1 (ACK-1) (EC 2.7.10.2) (EC 2.7.11.1) (Non-receptor protein tyrosine kinase Ack) (Tyrosine kinase non-receptor protein 2)
Gene: Tnk2

Interactor 2: NCK1 (Q99M51)
SH2/SH3 adapter protein Nck1 (Cytoplasmic protein Nck1) (NCK adapter protein 1) (Nck-1)
Gene: NCK1

Species: Mus musculus

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.44	1.00	0.665

Property	Details
Experiments	16777958
Identification Methods	affinity chromatography technology, anti bait coimmunoprecipitation
Interaction Type	physical association

Property	Details
Interacting Pfam domains	PK_Tyr_Ser-Thr~SH2, PK_Tyr_Ser-Thr~SH3_1
Common GO terms	GO:0005634 - nucleus GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0007165 - signal transduction
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 1055 Mm			1 377
+	Hs
		557670 ACK1_HUMAN (1-1055): 0: 831: 92.89		NCK1_HUMAN (1-377): 2.9e-97: 295: 98.94
+	Hs
		209039 ACK1_HUMAN (1-1055): 0: 831: 92.89		NCK2_HUMAN (1-376): 7.37e-90: 277: 68.50
+	Hs
		433331 ACK1_HUMAN (1-1055): 0: 831: 92.89		YES_HUMAN (6-152): 2.64e-22: 100: 19.87
+	Hs
		655334 ACK1_HUMAN (1-1055): 0: 831: 92.89		P85A_HUMAN (241-376): 2.35e-22: 100: 19.57
+	Hs
		412152 ACK1_HUMAN (1-1055): 0: 831: 92.89		FYN_HUMAN (6-130): 3.47e-22: 99.8: 21.26
+	Hs
		442817 ACK1_HUMAN (1-1055): 0: 831: 92.89		HCK_HUMAN (2-130): 1.23e-21: 98.3: 22.31
+	Hs
		522936 ACK1_HUMAN (1-1055): 0: 831: 92.89		SRC_HUMAN (6-130): 5.78e-19: 90.1: 21.26
+	Hs
		782781 ACK1_HUMAN (1-1055): 0: 831: 92.89		CSK_HUMAN (4-143): 8.06e-17: 83.7: 14.38
+	Hs
		924513 ACK1_HUMAN (1-1055): 0: 831: 92.89		GRB2_HUMAN (3-58): 7.33e-17: 80.6: 28.57
+	Mm
		337637 ABL1_MOUSE (2-994): 3.76e-160: 504: 21.40		NCK1_MOUSE (1-377): 8.52e-98: 297: 100.00
+	Hs
		337337 ABL1_HUMAN (2-994): 5.34e-148: 472: 20.16		NCK1_HUMAN (1-377): 2.9e-97: 295: 98.94
+	Dm
		993220 ACK_DROME (4-1047): 1.89e-164: 514: 31.79		GRAP_DROME (1-61): 5.38e-17: 80.7: 31.15
+	Hs
		298944 ABL1_HUMAN (2-994): 5.34e-148: 472: 20.16		SRBS2_HUMAN (49-287): 4.41e-23: 103: 16.74
+	Hs
		337356 ABL1_HUMAN (2-994): 5.34e-148: 472: 20.16		P85A_HUMAN (241-376): 2.35e-22: 100: 19.57
+	Hs
		337307 ABL1_HUMAN (2-994): 5.34e-148: 472: 20.16		FYN_HUMAN (6-130): 3.47e-22: 99.8: 21.26
+	Hs
		337315 ABL1_HUMAN (2-994): 5.34e-148: 472: 20.16		HCK_HUMAN (2-130): 1.23e-21: 98.3: 22.31
+	Hs
		129151 ABL1_HUMAN (2-994): 5.34e-148: 472: 20.16		P85B_HUMAN (252-376): 9.28e-20: 92.9: 24.41
+	Hs
		337329 ABL1_HUMAN (2-994): 5.34e-148: 472: 20.16		SRC_HUMAN (6-130): 5.78e-19: 90.1: 21.26
+	Hs
		337384 ABL1_HUMAN (2-994): 5.34e-148: 472: 20.16		ABL2_HUMAN (197-356): 8.37e-16: 81.3: 33.95
+	Hs
		337418 ABL1_HUMAN (2-994): 5.34e-148: 472: 20.16		GRB2_HUMAN (3-58): 7.33e-17: 80.6: 28.57