HitPredict

Interactor 1: NCK2 (O55033)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: EFNB1 (P52795)
Ephrin-B1 (CEK5 receptor ligand) (CEK5-L) (EFL-3) (ELK ligand) (ELK-L) (EPH-related receptor tyrosine kinase ligand 2) (LERK-2) (Stimulated by retinoic acid gene 1 protein) [Cleaved into: Ephrin-B1 C-terminal fragment (Ephrin-B1 CTF); Ephrin-B1 intracellular domain (Ephrin-B1 ICD)]
Gene: EFNB1

Species: Mus musculus

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.64	0.60	0.620

Property	Details
Experiments	11557983, 16511561
Identification Methods	affinity chromatography technology, two hybrid, anti bait coimmunoprecipitation
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0042102 - positive regulation of T cell proliferation GO:0045202 - synapse GO:0048013 - ephrin receptor signaling pathway
Homologous interactions	9

Homologous interactions

Color coding for hit scores

	1 380 Mm			1 345
+	Hs
		209030 NCK2_HUMAN (1-380): 1.15e-93: 287: 96.05		EFNB1_HUMAN (1-345): 0: 517: 95.67
+	Hs
		209015 NCK2_HUMAN (1-380): 1.15e-93: 287: 96.05		EFNB2_HUMAN (8-345): 4.74e-155: 439: 54.97
+	Mm
		223008 NCK2_MOUSE (1-380): 1.59e-93: 286: 100.00		EFNB2_MOUSE (2-345): 4.01e-155: 440: 53.16
+	Xl
		140380 Q6V1R3_XENLA (1-380): 9.8e-92: 282: 80.84		EFNB1_XENLA (26-345): 2.1e-150: 428: 75.00
+	Hs
		129276 P85B_HUMAN (2-379): 2.19e-30: 124: 12.53		EFNB1_HUMAN (1-345): 0: 517: 95.67
+	Hs
		522911 SRC_HUMAN (7-120): 5.83e-16: 81.3: 18.10		EFNB1_HUMAN (1-345): 0: 517: 95.67
+	Hs
		655281 P85A_HUMAN (24-379): 4.46e-31: 126: 12.57		EFNB2_HUMAN (8-345): 4.74e-155: 439: 54.97
+	Hs
		129251 P85B_HUMAN (2-379): 2.19e-30: 124: 12.53		EFNB2_HUMAN (8-345): 4.74e-155: 439: 54.97
+	Hs
		522869 SRC_HUMAN (7-120): 5.83e-16: 81.3: 18.10		EFNA5_HUMAN (14-204): 3.16e-69: 217: 24.51