HitPredict

Interactor 1: NCK2 (O55033)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: CBLB (Q3TTA7)
E3 ubiquitin-protein ligase CBL-B (EC 2.3.2.27) (Casitas B-lineage lymphoma proto-oncogene b) (RING-type E3 ubiquitin transferase CBL-B) (SH3-binding protein CBL-B) (Signal transduction protein CBL-B)
Gene: CBLB

Species: Mus musculus

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.33	0.60	0.445

Property	Details
Experiments	27474268
Identification Methods	affinity chromatography technology
Interaction Type	association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0001784 - phosphotyrosine residue binding GO:0005829 - cytosol GO:0007165 - signal transduction GO:0030971 - receptor tyrosine kinase binding
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 380 Mm			1 982
+	Hs
		209046 NCK2_HUMAN (1-380): 1.15e-93: 287: 96.05		CBLB_HUMAN (1-982): 0: 926: 94.30
+	Hs
		557678 NCK1_HUMAN (1-379): 1.12e-87: 271: 66.93		CBLB_HUMAN (1-982): 0: 926: 94.30
+	Mm
		82738 NCK1_MOUSE (1-379): 6.37e-88: 272: 67.19		B9EKI5_MOUSE (1-982): 0: 916: 90.53
+	Hs
		1027627 ITSN2_HUMAN (5-300): 7.23e-34: 135: 18.60		CBLB_HUMAN (1-982): 0: 926: 94.30
+	Hs
		655351 P85A_HUMAN (24-379): 4.46e-31: 126: 12.57		CBLB_HUMAN (1-982): 0: 926: 94.30
+	Hs
		129289 P85B_HUMAN (2-379): 2.19e-30: 124: 12.53		CBLB_HUMAN (1-982): 0: 926: 94.30
+	Hs
		1027606 ITSN1_HUMAN (4-305): 7.9e-24: 105: 15.78		CBLB_HUMAN (1-982): 0: 926: 94.30
+	Hs
		208994 NCK2_HUMAN (1-380): 1.15e-93: 287: 96.05		CBL_HUMAN (25-976): 0: 710: 47.24
+	Hs
		557607 NCK1_HUMAN (1-379): 1.12e-87: 271: 66.93		CBL_HUMAN (25-976): 0: 710: 47.24
+	Mm
		610633 NCK1_MOUSE (1-379): 6.37e-88: 272: 67.19		CBL_MOUSE (25-976): 0: 699: 46.94
+	Hs
		610517 ITSN2_HUMAN (5-300): 7.23e-34: 135: 18.60		CBL_HUMAN (25-976): 0: 710: 47.24
+	Hs
		610238 P85A_HUMAN (24-379): 4.46e-31: 126: 12.57		CBL_HUMAN (25-976): 0: 710: 47.24
+	Hs
		129202 P85B_HUMAN (2-379): 2.19e-30: 124: 12.53		CBL_HUMAN (25-976): 0: 710: 47.24
+	Mm
		140057 P85B_MOUSE (25-380): 8.91e-33: 131: 14.75		CBL_MOUSE (25-976): 0: 699: 46.94
+	Mm
		610546 P85A_MOUSE (24-379): 1.32e-31: 128: 12.57		CBL_MOUSE (25-976): 0: 699: 46.94
+	Hs
		610415 ITSN1_HUMAN (4-305): 7.9e-24: 105: 15.78		CBL_HUMAN (25-976): 0: 710: 47.24
+	Rn
		88780 P85A_RAT (24-379): 1.69e-30: 124: 12.57		D3ZV15_RAT (25-931): 0: 674: 47.36
+	Hs
		203208 NCK1_HUMAN (1-379): 1.12e-87: 271: 66.93		SYNJ1_HUMAN (419-879): 1.9e-13: 77.3: 15.60
+	Hs
		257713 ITSN2_HUMAN (5-300): 7.23e-34: 135: 18.60		ANR17_HUMAN (392-923): 3.35e-13: 76.6: 8.04
+	Hs
		203228 ITSN1_HUMAN (4-305): 7.9e-24: 105: 15.78		SYNJ1_HUMAN (419-879): 1.9e-13: 77.3: 15.60