HitPredict

Interactor 1: PRKN (O60260)
E3 ubiquitin-protein ligase parkin (Parkin) (EC 2.3.2.31) (Parkin RBR E3 ubiquitin-protein ligase) (Parkinson juvenile disease protein 2) (Parkinson disease protein 2)
Gene: PRKN

Interactor 2: RBP2 (P49792)
E3 SUMO-protein ligase RanBP2 (EC 2.3.2.-) (358 kDa nucleoporin) (Nuclear pore complex protein Nup358) (Nucleoporin Nup358) (Ran-binding protein 2) (RanBP2) (p270)
Gene: RANBP2

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.77	1.00	0.878

Property	Details
Experiments	16332688, 35509820, 35256949
Identification Methods	affinity chromatography technology, pull down, enzymatic study, two hybrid, anti tag coimmunoprecipitation, anti bait coimmunoprecipitation
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	ubiquitin~zf-RanBP
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0044877 - protein-containing complex binding
Homologous interactions	6

Homologous interactions

Color coding for hit scores

	1 465 Hs			1 3224
+	Rn
		88856 PRKN_RAT (1-465): 2.14e-136: 402: 85.16		D4A054_RAT (893-3224): 0: 1076: 61.48
+	Hs
		226540 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		PPIB_HUMAN (3021-3224): 3.73e-97: 314: 43.90
+	Hs
		227106 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		PPIA_HUMAN (3061-3224): 6.29e-96: 309: 65.85
+	Mm
		1322336 PRKN_MOUSE (1-465): 6.53e-131: 388: 83.66		RBP2_MOUSE (2382-3084): 2.76e-47: 191: 21.70
+	At
		713361 UBQ3_ARATH (1-77): 1.22e-30: 122: 31.17		CP20C_ARATH (3046-3224): 2.43e-94: 308: 52.22
+	Hs
		63871 UBC_HUMAN (1-77): 1.15e-29: 124: 32.47		RGPD3_HUMAN (2313-3076): 6.96e-42: 172: 16.92