HitPredict

Interactor 1: PRKN (O60260)
E3 ubiquitin-protein ligase parkin (Parkin) (EC 2.3.2.31) (Parkin RBR E3 ubiquitin-protein ligase) (Parkinson juvenile disease protein 2) (Parkinson disease protein 2)
Gene: PRKN

Interactor 2: FBXW4 (P57775)
F-box/WD repeat-containing protein 4 (Dactylin) (F-box and WD-40 domain-containing protein 4)
Gene: FBXW4

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.44	1.00	0.665

Property	Details
Experiments	32814053
Identification Methods	two hybrid array, two hybrid pooling approach, validated two hybrid
Interaction Type	physical association

Property	Details
Interacting Pfam domains	F-box-like~ubiquitin
Common GO terms	GO:0000151 - ubiquitin ligase complex GO:0005829 - cytosol GO:0006511 - ubiquitin-dependent protein catabolic process
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 465 Hs			1 412
+	Hs
		229023 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		FBW1B_HUMAN (34-411): 3.49e-81: 260: 16.03
+	Hs
		226765 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		LIS1_HUMAN (7-322): 9.15e-67: 219: 13.20
+	Hs
		227054 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		WDR5_HUMAN (1-319): 1.97e-65: 213: 12.61
+	Hs
		228276 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		FBXW7_HUMAN (24-276): 1.51e-43: 163: 14.89
+	Hs
		228555 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		KTNB1_HUMAN (100-275): 1.19e-32: 132: 15.47
+	Hs
		1356385 ARI1_HUMAN (163-464): 3.95e-74: 244: 19.67		FBW1B_HUMAN (34-411): 3.49e-81: 260: 16.03
+	Hs
		228868 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		NLE1_HUMAN (328-411): 2.65e-17: 85.6: 11.91
+	Hs
		228315 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		SNR40_HUMAN (322-411): 5.98e-14: 74.5: 14.29
+	Hs
		229098 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		FBW1A_HUMAN (39-151): 6.54e-10: 62.9: 10.83
+	Hs
		1281514 ARI1_HUMAN (163-464): 3.95e-74: 244: 19.67		FBXW7_HUMAN (24-276): 1.51e-43: 163: 14.89
+	Hs
		476547 UBC_HUMAN (1-77): 1.15e-29: 124: 32.47		FBW1B_HUMAN (34-411): 3.49e-81: 260: 16.03
+	Hs
		1219356 CUL9_HUMAN (30-463): 1.19e-80: 274: 18.58		SNR40_HUMAN (322-411): 5.98e-14: 74.5: 14.29
+	Hs
		475563 UBC_HUMAN (1-77): 1.15e-29: 124: 32.47		WDR5_HUMAN (1-319): 1.97e-65: 213: 12.61
+	Hs
		1377165 ARI1_HUMAN (163-464): 3.95e-74: 244: 19.67		FBW1A_HUMAN (39-151): 6.54e-10: 62.9: 10.83
+	Sc
		431108 UBI4P_YEAST (1-229): 3.48e-61: 205: 18.72		CDC4_YEAST (197-411): 4.7e-16: 82.2: 14.77
+	Hs
		476151 UBC_HUMAN (1-77): 1.15e-29: 124: 32.47		FBXW7_HUMAN (24-276): 1.51e-43: 163: 14.89
+	Sc
		477281 UBI4P_YEAST (1-229): 3.48e-61: 205: 18.72		MET30_YEAST (72-191): 5.44e-11: 66.4: 8.03
+	Sc
		477110 UBI4P_YEAST (1-229): 3.48e-61: 205: 18.72		TUP1_YEAST (160-275): 1.18e-10: 65.3: 24.82
+	Hs
		476181 UBC_HUMAN (1-77): 1.15e-29: 124: 32.47		SNR40_HUMAN (322-411): 5.98e-14: 74.5: 14.29
+	Hs
		476595 UBC_HUMAN (1-77): 1.15e-29: 124: 32.47		FBW1A_HUMAN (39-151): 6.54e-10: 62.9: 10.83