HitPredict

Interactor 1: PRKN (O60260)
E3 ubiquitin-protein ligase parkin (Parkin) (EC 2.3.2.31) (Parkin RBR E3 ubiquitin-protein ligase) (Parkinson juvenile disease protein 2) (Parkinson disease protein 2)
Gene: PRKN

Interactor 2: AP2B1 (P63010)
AP-2 complex subunit beta (AP105B) (Adaptor protein complex AP-2 subunit beta) (Adaptor-related protein complex 2 subunit beta) (Beta-2-adaptin) (Beta-adaptin) (Clathrin assembly protein complex 2 beta large chain) (Plasma membrane adaptor HA2/AP2 adaptin beta subunit)
Gene: AP2B1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.60	0.60	0.601

Property	Details
Experiments	35256949, 32814053
Identification Methods	affinity chromatography technology, two hybrid array, two hybrid pooling approach, validated two hybrid
Interaction Type	physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005829 - cytosol GO:0098793 - presynapse
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 465 Hs			1 937
+	Hs
		227287 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		AP1B1_HUMAN (1-937): 0: 888: 82.72
+	Hs
		475594 UBC_HUMAN (1-77): 1.15e-29: 124: 32.47		AP2B1_HUMAN (1-937): 0: 926: 100.00
+	Hs
		226166 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		AP2A1_HUMAN (11-932): 0: 634: 16.97
+	Hs
		226949 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		COPB_HUMAN (8-927): 0: 561: 12.47
+	Hs
		119893 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		AP3B1_HUMAN (8-594): 5.76e-178: 547: 28.17
+	Hs
		212576 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		AP1G1_HUMAN (11-808): 1.03e-173: 527: 17.57
+	At
		286688 UBQ3_ARATH (1-77): 1.22e-30: 122: 31.17		APBLC_ARATH (3-936): 0: 756: 56.46
+	Hs
		321678 ARI1_HUMAN (163-464): 3.95e-74: 244: 19.67		AP2A1_HUMAN (11-932): 0: 634: 16.97
+	At
		1092397 UBQ3_ARATH (1-77): 1.22e-30: 122: 31.17		APBLB_ARATH (3-936): 0: 750: 56.14
+	Hs
		302203 HOIL1_HUMAN (12-448): 5.59e-68: 227: 16.36		AP2A2_HUMAN (11-933): 0: 600: 16.99
+	Hs
		321470 UBC_HUMAN (1-77): 1.15e-29: 124: 32.47		AP2A1_HUMAN (11-932): 0: 634: 16.97
+	Hs
		302094 UBC_HUMAN (1-77): 1.15e-29: 124: 32.47		AP2A2_HUMAN (11-933): 0: 600: 16.99
+	Zm
		111864 Q42415_MAIZE (1-153): 8.84e-50: 179: 20.13		K7VAA4_MAIZE (13-808): 1.26e-178: 541: 15.95
+	Hs
		475514 UBC_HUMAN (1-77): 1.15e-29: 124: 32.47		COPB_HUMAN (8-927): 0: 561: 12.47
+	At
		1092398 UBQ3_ARATH (1-77): 1.22e-30: 122: 31.17		COPB1_ARATH (6-919): 0: 562: 13.63
+	At
		1091811 UBQ3_ARATH (1-77): 1.22e-30: 122: 31.17		AP2A2_ARATH (13-934): 0: 554: 15.90
+	Zm
		111863 Q41754_MAIZE (1-76): 8.11e-30: 121: 31.58		K7VAA4_MAIZE (13-808): 1.26e-178: 541: 15.95
+	At
		1013363 UBQ3_ARATH (1-77): 1.22e-30: 122: 31.17		COPG_ARATH (3-933): 4.18e-175: 533: 14.06
+	At
		1091731 UBQ3_ARATH (1-77): 1.22e-30: 122: 31.17		AP1G1_ARATH (14-809): 2.91e-173: 527: 16.34
+	At
		1092096 UBQ3_ARATH (1-77): 1.22e-30: 122: 31.17		APBLA_ARATH (12-741): 3.83e-165: 505: 30.21