HitPredict

Interactor 1: PRKN (O60260)
E3 ubiquitin-protein ligase parkin (Parkin) (EC 2.3.2.31) (Parkin RBR E3 ubiquitin-protein ligase) (Parkinson juvenile disease protein 2) (Parkinson disease protein 2)
Gene: PRKN

Interactor 2: DCAF8 (Q5TAQ9)
DDB1- and CUL4-associated factor 8 (WD repeat-containing protein 42A)
Gene: DCAF8

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.70	0.60	0.650

Property	Details
Experiments	35509820, 35256949, 32814053
Identification Methods	affinity chromatography technology, two hybrid array, two hybrid pooling approach, validated two hybrid
Interaction Type	physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0005739 - mitochondrion GO:0005829 - cytosol GO:0016567 - protein ubiquitination
Homologous interactions	16

Homologous interactions

Color coding for hit scores

	1 465 Hs			1 597
+	Hs
		228276 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		FBXW7_HUMAN (312-594): 7.67e-52: 191: 12.28
+	Hs
		228612 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		TBL1R_HUMAN (323-595): 1.19e-49: 181: 12.63
+	Hs
		228046 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		WDTC1_HUMAN (405-597): 4.23e-46: 174: 30.30
+	Hs
		228315 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		SNR40_HUMAN (83-358): 2.66e-47: 171: 14.69
+	Hs
		227054 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		WDR5_HUMAN (278-585): 2.83e-45: 165: 12.18
+	Hs
		227817 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		WDR82_HUMAN (329-546): 8.72e-13: 71.4: 10.90
+	Hs
		228868 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		NLE1_HUMAN (86-182): 4.74e-11: 67.2: 8.25
+	Hs
		227292 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		TBL3_HUMAN (76-182): 1.35e-09: 63: 9.35
+	Hs
		226765 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		LIS1_HUMAN (505-582): 6.91e-09: 60: 11.54
+	Hs
		1219356 CUL9_HUMAN (30-463): 1.19e-80: 274: 18.58		SNR40_HUMAN (83-358): 2.66e-47: 171: 14.69
+	Hs
		1281514 ARI1_HUMAN (163-464): 3.95e-74: 244: 19.67		FBXW7_HUMAN (312-594): 7.67e-52: 191: 12.28
+	Hs
		476211 UBC_HUMAN (1-77): 1.15e-29: 124: 32.47		DCAF5_HUMAN (153-542): 6e-79: 269: 22.72
+	Hs
		476151 UBC_HUMAN (1-77): 1.15e-29: 124: 32.47		FBXW7_HUMAN (312-594): 7.67e-52: 191: 12.28
+	Hs
		476181 UBC_HUMAN (1-77): 1.15e-29: 124: 32.47		SNR40_HUMAN (83-358): 2.66e-47: 171: 14.69
+	Hs
		475563 UBC_HUMAN (1-77): 1.15e-29: 124: 32.47		WDR5_HUMAN (278-585): 2.83e-45: 165: 12.18
+	At
		187391 UBQ3_ARATH (1-77): 1.22e-30: 122: 31.17		O22826_ARATH (417-591): 6.3e-29: 119: 13.48