HitPredict

Interactor 1: PRKN (O60260)
E3 ubiquitin-protein ligase parkin (Parkin) (EC 2.3.2.31) (Parkin RBR E3 ubiquitin-protein ligase) (Parkinson juvenile disease protein 2) (Parkinson disease protein 2)
Gene: PRKN

Interactor 2: UB2J1 (Q9Y385)
Ubiquitin-conjugating enzyme E2 J1 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme J1) (Non-canonical ubiquitin-conjugating enzyme 1) (NCUBE-1) (Yeast ubiquitin-conjugating enzyme UBC6 homolog E) (HsUBC6e)
Gene: UBE2J1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.88	1.00	0.943

Property	Details
Experiments	24647965, 11439185, 15603737, 12150907, 16862145, 32814053
Identification Methods	pull down, affinity chromatography technology, two hybrid array, two hybrid pooling approach, validated two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	UQ_con~ubiquitin, UQ_con~zf-RING_14
Common GO terms	GO:0000209 - protein polyubiquitination GO:0005634 - nucleus GO:0031625 - ubiquitin protein ligase binding GO:0036503 - ERAD pathway
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 465 Hs			1 318
+	Hs
		227093 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		UB2D2_HUMAN (10-156): 5.15e-70: 216: 28.00
+	Hs
		229121 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		UB2D4_HUMAN (10-156): 3.97e-70: 216: 28.00
+	Hs
		226914 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		UB2D1_HUMAN (10-156): 1e-69: 215: 30.00
+	Hs
		227029 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		UB2D3_HUMAN (10-156): 1.73e-68: 212: 28.00
+	Hs
		226922 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		UB2E1_HUMAN (1-156): 1.67e-64: 203: 28.30
+	Hs
		228034 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		UB2J2_HUMAN (1-150): 3.16e-63: 202: 39.87
+	Hs
		228283 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		UB2E3_HUMAN (2-156): 9.16e-63: 199: 26.58
+	Hs
		228387 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		UB2E2_HUMAN (2-156): 2.19e-62: 198: 27.22
+	Hs
		226844 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		UBE2A_HUMAN (7-154): 3.69e-61: 193: 21.85
+	Hs
		227115 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		UBE2B_HUMAN (7-154): 6.08e-60: 190: 22.52
+	Hs
		227016 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		UB2G2_HUMAN (7-142): 5.84e-58: 185: 26.00
+	Hs
		227065 PRKN_HUMAN (1-465): 7.37e-137: 403: 100.00		UB2G1_HUMAN (15-148): 2.26e-56: 182: 25.83
+	Dm
		1182262 PRKN_DROME (1-463): 3.5e-106: 325: 42.19		Q9VEJ4_DROME (10-148): 1.09e-60: 196: 42.14
+	Hs
		849498 ARI1_HUMAN (163-464): 3.95e-74: 244: 19.67		UB2D1_HUMAN (10-156): 1e-69: 215: 30.00
+	Hs
		895968 ARI1_HUMAN (163-464): 3.95e-74: 244: 19.67		UB2D3_HUMAN (10-156): 1.73e-68: 212: 28.00
+	Hs
		908261 CUL9_HUMAN (30-463): 1.19e-80: 274: 18.58		UB2G1_HUMAN (15-148): 2.26e-56: 182: 25.83
+	Dm
		639397 LUBEL_DROME (55-453): 1.14e-66: 234: 16.95		UBCD1_DROME (10-156): 3.06e-69: 214: 29.33
+	Ce
		715114 Q9XUS3_CAEEL (6-464): 2.19e-70: 230: 25.44		UBC2_CAEEL (10-156): 1.22e-69: 214: 28.67
+	Hs
		1297383 ARI1_HUMAN (163-464): 3.95e-74: 244: 19.67		UB2E2_HUMAN (2-156): 2.19e-62: 198: 27.22
+	Ce
		1341608 Q9XUS3_CAEEL (6-464): 2.19e-70: 230: 25.44		Q9N490_CAEEL (6-150): 1.63e-62: 199: 41.10