HitPredict

Interactor 1: NUAK1 (O60285)
NUAK family SNF1-like kinase 1 (EC 2.7.11.1) (AMPK-related protein kinase 5) (ARK5) (Omphalocele kinase 1)
Gene: NUAK1

Interactor 2: 1433B (P31946)
14-3-3 protein beta/alpha (Protein 1054) (Protein kinase C inhibitor protein 1) (KCIP-1) [Cleaved into: 14-3-3 protein beta/alpha, N-terminally processed]
Gene: YWHAB

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.47	0.60	0.536

Property	Details
Experiments	32707033
Identification Methods	affinity chromatography technology, pull down
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 661 Hs			1 246
+	Hs
		229938 NUAK1_HUMAN (1-661): 0: 581: 100.00		1433E_HUMAN (3-243): 4.39e-144: 405: 65.43
+	Mm
		910266 NUAK1_MOUSE (1-661): 0: 557: 90.79		1433E_MOUSE (3-243): 4.39e-144: 405: 65.43
+	Hs
		229936 NUAK1_HUMAN (1-661): 0: 581: 100.00		1433G_HUMAN (3-246): 1.91e-109: 317: 75.40
+	Hs
		229942 NUAK1_HUMAN (1-661): 0: 581: 100.00		1433F_HUMAN (4-244): 2.7e-107: 311: 75.51
+	Xl
		223344 Q804T2_XENLA (4-658): 1.2e-161: 483: 25.67		O57468_XENLA (4-243): 2.71e-143: 402: 66.12
+	Hs
		647862 MARK3_HUMAN (4-658): 1.17e-156: 471: 27.09		1433E_HUMAN (3-243): 4.39e-144: 405: 65.43
+	Hs
		647835 MARK3_HUMAN (4-658): 1.17e-156: 471: 27.09		1433B_HUMAN (1-246): 3.48e-144: 404: 100.00
+	Mm
		910231 MARK3_MOUSE (4-658): 2.75e-156: 470: 27.09		1433E_MOUSE (3-243): 4.39e-144: 405: 65.43
+	Mm
		910308 MARK4_MOUSE (4-655): 2.73e-155: 468: 27.01		1433E_MOUSE (3-243): 4.39e-144: 405: 65.43
+	Hs
		647869 MARK3_HUMAN (4-658): 1.17e-156: 471: 27.09		1433Z_HUMAN (3-244): 1.49e-141: 398: 87.60
+	Mm
		926780 MARK3_MOUSE (4-658): 2.75e-156: 470: 27.09		1433Z_MOUSE (3-244): 3.14e-142: 399: 88.02
+	Mm
		975157 MARK3_MOUSE (4-658): 2.75e-156: 470: 27.09		1433B_MOUSE (1-246): 5.56e-141: 396: 98.78
+	Hs
		646650 MARK3_HUMAN (4-658): 1.17e-156: 471: 27.09		1433T_HUMAN (3-244): 3.24e-127: 361: 81.82
+	Hs
		647860 MARK3_HUMAN (4-658): 1.17e-156: 471: 27.09		1433G_HUMAN (3-246): 1.91e-109: 317: 75.40
+	Mm
		905331 MARK3_MOUSE (4-658): 2.75e-156: 470: 27.09		1433G_MOUSE (3-246): 1.91e-109: 317: 75.40
+	Hs
		904994 MARK4_HUMAN (4-655): 1.51e-155: 468: 27.16		1433G_HUMAN (3-246): 1.91e-109: 317: 75.40
+	Hs
		647879 MARK3_HUMAN (4-658): 1.17e-156: 471: 27.09		1433F_HUMAN (4-244): 2.7e-107: 311: 75.51
+	Mm
		935481 MARK3_MOUSE (4-658): 2.75e-156: 470: 27.09		1433F_MOUSE (4-244): 8.67e-108: 312: 75.51
+	Mm
		935569 MARK4_MOUSE (4-655): 2.73e-155: 468: 27.01		1433F_MOUSE (4-244): 8.67e-108: 312: 75.51
+	Hs
		647836 MARK3_HUMAN (4-658): 1.17e-156: 471: 27.09		1433S_HUMAN (3-239): 3.38e-100: 293: 68.62