HitPredict

Interactor 1: JAK2 (O60674)
Tyrosine-protein kinase JAK2 (EC 2.7.10.2) (Janus kinase 2) (JAK-2)
Gene: JAK2

Interactor 2: SH2B1 (Q9NRF2)
SH2B adapter protein 1 (Pro-rich, PH and SH2 domain-containing signaling mediator) (PSM) (SH2 domain-containing protein 1B)
Gene: SH2B1

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.87	1.00	0.934

Property	Details
Experiments	15316008, 9343427, 15767667, 12370803, 17565041
Identification Methods	affinity chromatography technology, two hybrid, pull down, coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	FERM_F1~SH2, PH~PK_Tyr_Ser-Thr, PK_Tyr_Ser-Thr~SH2, SH2~SH2
Common GO terms	GO:0005634 - nucleus GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0035556 - intracellular signal transduction GO:0060391 - positive regulation of SMAD protein signal transduction
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 1132 Hs			1 756
+	Rn
		1144006 JAK2_RAT (1-1131): 0: 1124: 94.70		SH2B1_RAT (1-756): 7.24e-167: 501: 90.63
+	Mm
		1143573 JAK2_MOUSE (1-1129): 0: 1108: 93.89		SH2B1_MOUSE (1-756): 2.52e-167: 502: 91.16
+	Hs
		145961 JAK2_HUMAN (1-1132): 0: 1122: 100.00		SH2B2_HUMAN (25-755): 5.52e-128: 396: 35.11
+	Hs
		238363 JAK2_HUMAN (1-1132): 0: 1122: 100.00		SHC1_HUMAN (52-627): 2.87e-100: 322: 17.54
+	Mm
		957236 JAK2_MOUSE (1-1129): 0: 1108: 93.89		SHC1_MOUSE (52-627): 1.88e-100: 323: 17.07
+	Mm
		1143584 JAK3_MOUSE (18-1132): 0: 927: 46.64		SH2B1_MOUSE (1-756): 2.52e-167: 502: 91.16
+	Hs
		238405 JAK2_HUMAN (1-1132): 0: 1122: 100.00		GRB10_HUMAN (82-618): 1.38e-92: 302: 12.05
+	Hs
		238345 JAK2_HUMAN (1-1132): 0: 1122: 100.00		VAV_HUMAN (40-685): 2.92e-87: 295: 13.46
+	Hs
		238330 JAK2_HUMAN (1-1132): 0: 1122: 100.00		FYN_HUMAN (401-742): 2.99e-74: 252: 13.87
+	Hs
		238352 JAK2_HUMAN (1-1132): 0: 1122: 100.00		PLCG1_HUMAN (451-753): 3.41e-69: 249: 17.19
+	Hs
		238446 JAK2_HUMAN (1-1132): 0: 1122: 100.00		SH2B3_HUMAN (340-754): 1.95e-72: 248: 30.12
+	Hs
		238342 JAK2_HUMAN (1-1132): 0: 1122: 100.00		SRC_HUMAN (376-742): 1.26e-71: 245: 13.88
+	Mm
		403446 JAK2_MOUSE (1-1129): 0: 1108: 93.89		SRC_MOUSE (376-742): 2.81e-73: 249: 13.68
+	Mm
		980968 JAK2_MOUSE (1-1129): 0: 1108: 93.89		YES_MOUSE (356-742): 2.79e-72: 247: 14.00
+	Mm
		140162 JAK2_MOUSE (1-1129): 0: 1108: 93.89		SH2B3_MOUSE (480-754): 5.89e-63: 222: 38.08
+	Hs
		238350 JAK2_HUMAN (1-1132): 0: 1122: 100.00		PLCG2_HUMAN (507-753): 4.8e-47: 183: 17.11
+	Hs
		238395 JAK2_HUMAN (1-1132): 0: 1122: 100.00		GRB2_HUMAN (517-646): 2.75e-16: 80.5: 18.46
+	Hs
		548650 TYK2_HUMAN (38-1132): 0: 870: 35.49		VAV_HUMAN (40-685): 2.92e-87: 295: 13.46
+	Hs
		412039 TYK2_HUMAN (38-1132): 0: 870: 35.49		FYN_HUMAN (401-742): 2.99e-74: 252: 13.87
+	Hs
		412070 ABL2_HUMAN (821-1129): 1.95e-95: 332: 31.61		FYN_HUMAN (401-742): 2.99e-74: 252: 13.87