HitPredict

Interactor 1: BRE1B (O75150)
E3 ubiquitin-protein ligase BRE1B (BRE1-B) (EC 2.3.2.27) (95 kDa retinoblastoma-associated protein) (RBP95) (RING finger protein 40) (RING-type E3 ubiquitin transferase BRE1B)
Gene: RNF40

Interactor 2: UBE2A (P49459)
Ubiquitin-conjugating enzyme E2 A (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme A) (RAD6 homolog A) (HR6A) (hHR6A) (Ubiquitin carrier protein A) (Ubiquitin-protein ligase A)
Gene: UBE2A

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.89	1.00	0.944

Property	Details
Experiments	25582440, 28031328, 19410543, 35271311, 37633270, 24837678
Identification Methods	affinity chromatography technology, pull down, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	UQ_con~zf-C3HC4
Common GO terms	GO:0005654 - nucleoplasm GO:0006511 - ubiquitin-dependent protein catabolic process GO:0031625 - ubiquitin protein ligase binding GO:0033503 - HULC complex
Homologous interactions	16

Homologous interactions

Color coding for hit scores

	1 1001 Hs			1 152
+	At
		639386 BRE1A_ARATH (497-1000): 3.93e-99: 334: 23.12		UBC1_ARATH (1-150): 1.31e-73: 218: 76.67
+	At
		790156 BRE1A_ARATH (497-1000): 3.93e-99: 334: 23.12		UBC2_ARATH (1-150): 1.51e-73: 218: 76.00
+	Hs
		256237 BRE1B_HUMAN (1-1001): 5.92e-75: 268: 100.00		UB2D2_HUMAN (5-149): 7.73e-78: 229: 38.62
+	Hs
		256239 BRE1B_HUMAN (1-1001): 5.92e-75: 268: 100.00		UBE2B_HUMAN (1-151): 3.28e-74: 219: 96.03
+	Hs
		256229 BRE1B_HUMAN (1-1001): 5.92e-75: 268: 100.00		UB2E1_HUMAN (3-150): 5.05e-71: 213: 36.49
+	Hs
		919748 BRE1A_HUMAN (1-1000): 8.12e-65: 238: 56.49		UB2D2_HUMAN (5-149): 7.73e-78: 229: 38.62
+	Hs
		829017 BRE1A_HUMAN (1-1000): 8.12e-65: 238: 56.49		UBE2A_HUMAN (1-152): 3.88e-76: 225: 100.00
+	Hs
		927757 BRE1A_HUMAN (1-1000): 8.12e-65: 238: 56.49		UBE2B_HUMAN (1-151): 3.28e-74: 219: 96.03
+	Hs
		851821 BRE1A_HUMAN (1-1000): 8.12e-65: 238: 56.49		UB2E1_HUMAN (3-150): 5.05e-71: 213: 36.49
+	Sp
		251716 BRL2_SCHPO (646-1000): 1.91e-60: 221: 21.23		UBC2_SCHPO (1-150): 2.83e-70: 209: 72.00
+	Sc
		410240 BRE1_YEAST (506-1000): 2.2e-57: 212: 18.77		UBC2_YEAST (1-150): 2.29e-73: 218: 69.33
+	Sp
		618356 BRL1_SCHPO (504-1000): 9.54e-26: 116: 18.57		UBC2_SCHPO (1-150): 2.83e-70: 209: 72.00
+	Ce
		712245 BRE1_CAEEL (25-799): 1.9e-14: 79.9: 15.44		UBC2_CAEEL (5-149): 9.01e-78: 228: 38.62
+	At
		639387 BRE1B_ARATH (38-843): 1.3e-15: 83.9: 12.39		UBC1_ARATH (1-150): 1.31e-73: 218: 76.67
+	At
		790157 BRE1B_ARATH (38-843): 1.3e-15: 83.9: 12.39		UBC2_ARATH (1-150): 1.51e-73: 218: 76.00
+	Ce
		712246 BRE1_CAEEL (25-799): 1.9e-14: 79.9: 15.44		UBC1_CAEEL (1-149): 2.14e-72: 216: 85.91