HitPredict

Interactor 1: EPN1 (O88339)
Epsin-1 (EPS-15-interacting protein 1)
Gene: EPN1

Interactor 2: AP2A2 (P18484)
AP-2 complex subunit alpha-2 (100 kDa coated vesicle protein C) (Adaptor protein complex AP-2 subunit alpha-2) (Adaptor-related protein complex 2 subunit alpha-2) (Alpha-adaptin C) (Alpha2-adaptin) (Clathrin assembly protein complex 2 alpha-C large chain) (Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit)
Gene: AP2A2

Species: Rattus norvegicus

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.74	0.60	0.670

Property	Details
Experiments	9723620, 10692452, 12057195
Identification Methods	pull down, far western blotting, anti bait coimmunoprecipitation
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005886 - plasma membrane GO:0035615 - clathrin adaptor activity
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 575 Rn			1 938
+	Rn
		291395 EPN1_RAT (1-575): 2.97e-96: 306: 100.00		Q66HM2_RAT (1-938): 0: 890: 99.57
+	Hs
		302230 EPN1_HUMAN (1-575): 2e-90: 291: 94.44		AP2A2_HUMAN (1-938): 0: 872: 96.06
+	Rn
		88757 EPN1_RAT (1-575): 2.97e-96: 306: 100.00		D3ZUY8_RAT (1-938): 0: 835: 80.81
+	Hs
		302148 EPN4_HUMAN (3-560): 2.7e-80: 266: 25.43		AP2A2_HUMAN (1-938): 0: 872: 96.06
+	Hs
		321680 EPN1_HUMAN (1-575): 2e-90: 291: 94.44		AP2A1_HUMAN (1-938): 0: 834: 78.37
+	Hs
		321545 EPN4_HUMAN (3-560): 2.7e-80: 266: 25.43		AP2A1_HUMAN (1-938): 0: 834: 78.37
+	Rn
		291389 EPN1_RAT (1-575): 2.97e-96: 306: 100.00		AP2B1_RAT (36-933): 0: 628: 16.58
+	At
		1228257 EPN1_ARATH (2-448): 1.01e-65: 226: 21.35		AP2A1_ARATH (8-938): 0: 705: 37.31
+	Hs
		1014105 EPN1_HUMAN (1-575): 2e-90: 291: 94.44		AP1B1_HUMAN (36-935): 0: 631: 16.99
+	Hs
		926065 EPN1_HUMAN (1-575): 2e-90: 291: 94.44		AP2B1_HUMAN (36-933): 0: 628: 16.58
+	Hs
		1014011 EPN4_HUMAN (3-560): 2.7e-80: 266: 25.43		AP1B1_HUMAN (36-935): 0: 631: 16.99
+	Hs
		925867 EPN4_HUMAN (3-560): 2.7e-80: 266: 25.43		AP2B1_HUMAN (36-933): 0: 628: 16.58
+	Dm
		1096902 Q8IN05_DROME (3-430): 5.86e-87: 296: 27.01		Q24253_DROME (36-931): 0: 592: 15.77
+	Hs
		212629 EPN4_HUMAN (3-560): 2.7e-80: 266: 25.43		AP1G1_HUMAN (1-823): 0: 610: 24.70
+	Rn
		923862 EPN2_RAT (1-572): 2.92e-65: 225: 43.17		AP2B1_RAT (36-933): 0: 628: 16.58
+	Dm
		1096905 Q9VD13_DROME (3-566): 5.49e-67: 231: 22.50		Q24253_DROME (36-931): 0: 592: 15.77
+	Sc
		743416 ENT1_YEAST (3-225): 5.58e-63: 215: 37.22		AP2A_YEAST (10-907): 0: 598: 26.47
+	At
		1195469 EPN1_ARATH (2-448): 1.01e-65: 226: 21.35		AP1G1_ARATH (1-809): 2.12e-180: 546: 23.43
+	Sc
		812546 ENT3_YEAST (4-428): 3.5e-73: 241: 24.59		AP1G1_YEAST (13-459): 4.47e-24: 110: 23.69
+	Sc
		812543 ENT3_YEAST (4-428): 3.5e-73: 241: 24.59		AP3D_YEAST (51-603): 1.51e-19: 96.7: 21.11