HitPredict

Interactor 1: GBRAP (O95166)
Gamma-aminobutyric acid receptor-associated protein (GABA(A) receptor-associated protein) (MM46)
Gene: GABARAP

Interactor 2: ATG4B (Q9Y4P1)
Cysteine protease ATG4B (EC 3.4.22.-) (AUT-like 1 cysteine endopeptidase) (Autophagy-related cysteine endopeptidase 1) (Autophagin-1) (Autophagy-related protein 4 homolog B) (HsAPG4B) (hAPG4B)
Gene: ATG4B

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.94	1.00	0.973

Property	Details
Experiments	28287329, 31625269, 33961781, 20562859, 25126726, 16169070, 15187094
Identification Methods	pull down, affinity chromatography technology, enzymatic study, two hybrid, protease assay, anti tag coimmunoprecipitation, two hybrid pooling approach
Interaction Type	direct interaction, physical association, protein cleavage, association

Property	Details
Interacting Pfam domains	ATG8~Peptidase_C54
Common GO terms	GO:0000045 - autophagosome assembly GO:0000421 - autophagosome membrane GO:0005829 - cytosol GO:0015031 - protein transport GO:0031410 - cytoplasmic vesicle
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 117 Hs			1 393
+	Hs
		891370 GBRL2_HUMAN (1-117): 2.75e-58: 176: 57.27		ATG4B_HUMAN (1-393): 0: 634: 100.00
+	Hs
		1328075 GBRL1_HUMAN (1-116): 8.15e-56: 170: 87.07		ATG4B_HUMAN (1-393): 0: 634: 100.00
+	Hs
		1330686 MLP3A_HUMAN (5-117): 2.29e-45: 144: 30.44		ATG4B_HUMAN (1-393): 0: 634: 100.00
+	Mm
		1206409 MLP3A_MOUSE (5-117): 2.29e-45: 144: 30.44		ATG4B_MOUSE (1-393): 0: 630: 94.91
+	Hs
		891248 GBRL2_HUMAN (1-117): 2.75e-58: 176: 57.27		ATG4A_HUMAN (10-368): 2.51e-171: 485: 55.53
+	Hs
		306253 GBRAP_HUMAN (1-117): 8.06e-57: 173: 100.00		ATG4A_HUMAN (10-368): 2.51e-171: 485: 55.53
+	Hs
		1264549 GBRL1_HUMAN (1-116): 8.15e-56: 170: 87.07		ATG4A_HUMAN (10-368): 2.51e-171: 485: 55.53
+	Ce
		111920 LGG1_CAEEL (1-116): 2.47e-55: 170: 83.62		ATG41_CAEEL (5-393): 7.98e-166: 475: 37.64
+	Hs
		1264550 MLP3A_HUMAN (5-117): 2.29e-45: 144: 30.44		ATG4A_HUMAN (10-368): 2.51e-171: 485: 55.53
+	Hs
		891203 GBRL2_HUMAN (1-117): 2.75e-58: 176: 57.27		ATG4D_HUMAN (24-391): 4.43e-155: 447: 30.58
+	Hs
		306219 GBRAP_HUMAN (1-117): 8.06e-57: 173: 100.00		ATG4D_HUMAN (24-391): 4.43e-155: 447: 30.58
+	Hs
		1198603 GBRL1_HUMAN (1-116): 8.15e-56: 170: 87.07		ATG4D_HUMAN (24-391): 4.43e-155: 447: 30.58
+	Dm
		1372164 Q9W2S2_DROME (1-117): 5.29e-58: 176: 91.45		Q9VPW2_DROME (14-358): 5.47e-133: 388: 44.13
+	Dm
		114224 Q9VEG5_DROME (1-116): 5.35e-54: 166: 77.59		M9PBM3_DROME (14-358): 4.75e-133: 389: 44.13
+	Dm
		1179054 Q7K1J3_DROME (1-116): 4.56e-54: 167: 77.59		Q9VPW2_DROME (14-358): 5.47e-133: 388: 44.13
+	Dm
		1364631 Q9VEG5_DROME (1-116): 5.35e-54: 166: 77.59		Q9VPW2_DROME (14-358): 5.47e-133: 388: 44.13
+	Hs
		306269 GBRAP_HUMAN (1-117): 8.06e-57: 173: 100.00		ATG4C_HUMAN (22-391): 2.09e-127: 376: 24.94
+	Hs
		1288559 GBRL1_HUMAN (1-116): 8.15e-56: 170: 87.07		ATG4C_HUMAN (22-391): 2.09e-127: 376: 24.94
+	Ce
		999365 LGG1_CAEEL (1-116): 2.47e-55: 170: 83.62		ATG42_CAEEL (25-355): 2.36e-108: 329: 29.47
+	Sc
		746022 ATG8_YEAST (1-116): 9.18e-59: 178: 55.17		ATG4_YEAST (28-342): 6.14e-93: 289: 27.75