HitPredict

Interactor 1: AP2A1 (O95782)
AP-2 complex subunit alpha-1 (100 kDa coated vesicle protein A) (Adaptor protein complex AP-2 subunit alpha-1) (Adaptor-related protein complex 2 subunit alpha-1) (Alpha-adaptin A) (Alpha1-adaptin) (Clathrin assembly protein complex 2 alpha-A large chain) (Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit)
Gene: AP2A1

Interactor 2: SHC1 (P29353)
SHC-transforming protein 1 (SHC-transforming protein 3) (SHC-transforming protein A) (Src homology 2 domain-containing-transforming protein C1) (SH2 domain protein C1)
Gene: SHC1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.90	0.60	0.735

Property	Details
Experiments	24189400, 28514442, 31980649, 33961781, 19380743, unassigned2173
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation, tandem affinity purification
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005829 - cytosol GO:0005886 - plasma membrane
Homologous interactions	17

Homologous interactions

Color coding for hit scores

	1 977 Hs			1 583
+	Hs
		302103 AP2A2_HUMAN (1-977): 0: 832: 76.97		SHC1_HUMAN (1-583): 0: 531: 100.00
+	Hs
		321527 AP2A1_HUMAN (1-977): 0: 904: 100.00		SHC2_HUMAN (56-582): 4.56e-116: 358: 44.88
+	Hs
		666058 AP2B1_HUMAN (34-972): 0: 676: 17.11		SHC1_HUMAN (1-583): 0: 531: 100.00
+	Hs
		666090 AP1B1_HUMAN (34-972): 0: 668: 18.15		SHC1_HUMAN (1-583): 0: 531: 100.00
+	Hs
		321544 AP2A1_HUMAN (1-977): 0: 904: 100.00		GRB7_HUMAN (371-563): 3.91e-33: 135: 21.57
+	Hs
		957123 AP1B1_HUMAN (34-972): 0: 668: 18.15		SHC2_HUMAN (56-582): 4.56e-116: 358: 44.88
+	Mm
		567529 AP2A2_MOUSE (1-977): 0: 841: 79.29		ANS1B_MOUSE (39-364): 1.02e-45: 175: 17.86
+	Hs
		321522 AP2A1_HUMAN (1-977): 0: 904: 100.00		GRB2_HUMAN (482-578): 1.65e-15: 77.5: 30.48
+	Dm
		561275 AP2A_DROME (3-977): 0: 784: 66.09		NUMB_DROME (117-466): 1.02e-43: 165: 15.86
+	Ce
		283751 Q22601_CAEEL (1-977): 0: 768: 60.49		CED6_CAEEL (123-481): 2.83e-45: 169: 15.10
+	Hs
		302130 AP2A2_HUMAN (1-977): 0: 832: 76.97		GRB2_HUMAN (482-578): 1.65e-15: 77.5: 30.48
+	Hs
		925962 AP2B1_HUMAN (34-972): 0: 676: 17.11		ANS1A_HUMAN (40-393): 2.28e-42: 165: 18.41
+	Ce
		283753 Q9N4F3_CAEEL (34-974): 0: 603: 17.15		CED6_CAEEL (123-481): 2.83e-45: 169: 15.10
+	Hs
		924449 AP2B1_HUMAN (34-972): 0: 676: 17.11		GRB2_HUMAN (482-578): 1.65e-15: 77.5: 30.48
+	Mm
		193241 AP1B1_MOUSE (34-972): 0: 657: 17.61		GRB2_MOUSE (482-578): 3.54e-15: 76.8: 30.48
+	Dm
		561280 Q7KVR8_DROME (12-681): 1.63e-151: 475: 26.01		NUMB_DROME (117-466): 1.02e-43: 165: 15.86
+	Hs
		150192 AP3D1_HUMAN (11-746): 3.68e-150: 476: 20.55		BMX_HUMAN (341-582): 1.11e-38: 153: 19.69