HitPredict

Interactor 1: M4K4 (O95819)
Mitogen-activated protein kinase kinase kinase kinase 4 (EC 2.7.11.1) (HPK/GCK-like kinase HGK) (MAPK/ERK kinase kinase kinase 4) (MEK kinase kinase 4) (MEKKK 4) (Nck-interacting kinase)
Gene: MAP4K4

Interactor 2: NCK1 (P16333)
SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)
Gene: NCK1

Species: Homo sapiens

Source: biogrid

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.64	0.60	0.624

Property	Details
Experiments	9135144, 32640226
Identification Methods	pull down, affinity chromatography technology
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 1239 Hs			1 377
+	Hs
		208968 M4K4_HUMAN (1-1239): 0: 1227: 100.00		NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24
+	Mm
		956970 M4K4_MOUSE (1-1239): 0: 1142: 88.17		NCK1_MOUSE (1-377): 7.48e-99: 300: 98.94
+	Hs
		557740 MINK1_HUMAN (1-1239): 0: 1120: 59.29		NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00
+	Hs
		557820 TNIK_HUMAN (1-1239): 0: 1101: 60.56		NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00
+	Hs
		209112 MINK1_HUMAN (1-1239): 0: 1120: 59.29		NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24
+	Hs
		209186 TNIK_HUMAN (1-1239): 0: 1101: 60.56		NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24
+	Hs
		324782 M4K4_HUMAN (1-1239): 0: 1227: 100.00		GRB2_HUMAN (3-61): 2.12e-16: 79.1: 28.81
+	Mm
		949279 TNIK_MOUSE (1-1239): 0: 1120: 62.43		SRBS2_MOUSE (46-288): 1.38e-24: 107: 18.85
+	Hs
		924703 MINK1_HUMAN (1-1239): 0: 1120: 59.29		GRB2_HUMAN (3-61): 2.12e-16: 79.1: 28.81
+	Dm
		1182596 Q7KV88_DROME (3-813): 1.25e-117: 395: 40.57		NCK_DROME (5-374): 5.6e-68: 225: 42.54
+	Hs
		148235 CTRO_HUMAN (14-616): 3.12e-117: 405: 22.33		SRBS2_HUMAN (64-287): 1.79e-23: 104: 18.26
+	Hs
		298951 AKT2_HUMAN (2-354): 7.38e-124: 393: 21.98		SRBS2_HUMAN (64-287): 1.79e-23: 104: 18.26
+	Hs
		782836 MAST1_HUMAN (3-947): 3.96e-126: 426: 14.68		CSK_HUMAN (4-143): 1.92e-12: 70.2: 14.38
+	Mm
		643056 AKT1_MOUSE (2-354): 1.36e-122: 389: 20.82		P85A_MOUSE (241-376): 1.59e-22: 101: 19.57
+	Hs
		655233 AKT1_HUMAN (2-354): 2.35e-122: 388: 20.82		P85A_HUMAN (241-376): 1.37e-22: 101: 19.57
+	Hs
		412167 AAPK1_HUMAN (8-555): 9.95e-123: 392: 16.85		FYN_HUMAN (7-173): 7.47e-21: 96: 15.39
+	Hs
		412046 AKT1_HUMAN (2-354): 2.35e-122: 388: 20.82		FYN_HUMAN (7-173): 7.47e-21: 96: 15.39
+	Dm
		993188 Q7KV89_DROME (3-813): 4.51e-117: 393: 40.32		GRAP_DROME (1-61): 1.53e-17: 82.3: 31.15
+	Mm
		403418 AKT1_MOUSE (2-354): 1.36e-122: 389: 20.82		SRC_MOUSE (5-114): 1.55e-15: 79.7: 22.32
+	Hs
		522810 AKT1_HUMAN (2-354): 2.35e-122: 388: 20.82		SRC_HUMAN (5-114): 1.61e-15: 79.7: 22.32