HitPredict

Interactor 1: M4K4 (O95819)
Mitogen-activated protein kinase kinase kinase kinase 4 (EC 2.7.11.1) (HPK/GCK-like kinase HGK) (MAPK/ERK kinase kinase kinase 4) (MEK kinase kinase 4) (MEKKK 4) (Nck-interacting kinase)
Gene: MAP4K4

Interactor 2: 2AAA (P30153)
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform (Medium tumor antigen-associated 61 kDa protein) (PP2A subunit A isoform PR65-alpha) (PP2A subunit A isoform R1-alpha)
Gene: PPP2R1A

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.47	1.00	0.691

Property	Details
Experiments	35271311
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	HEAT~Pkinase, HEAT_2~Pkinase
Common GO terms	GO:0005737 - cytoplasm GO:0035556 - intracellular signal transduction
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 1239 Hs			1 589
+	Mm
		949335 TNIK_MOUSE (1-1239): 0: 1120: 62.43		2AAA_MOUSE (1-589): 2.29e-166: 488: 99.83
+	Hs
		672343 TNIK_HUMAN (1-1239): 0: 1101: 60.56		2AAA_HUMAN (1-589): 2.84e-166: 487: 100.00
+	Hs
		672353 MAST1_HUMAN (3-947): 3.96e-126: 426: 14.68		2AAA_HUMAN (1-589): 2.84e-166: 487: 100.00
+	Hs
		647833 MARK3_HUMAN (16-665): 4.1e-127: 411: 16.23		2AAA_HUMAN (1-589): 2.84e-166: 487: 100.00
+	Hs
		148432 CTRO_HUMAN (14-616): 3.12e-117: 405: 22.33		2AAA_HUMAN (1-589): 2.84e-166: 487: 100.00
+	Hs
		672494 MAST1_HUMAN (3-947): 3.96e-126: 426: 14.68		2AAB_HUMAN (9-589): 7.23e-158: 466: 86.40
+	Hs
		672244 MARK4_HUMAN (16-693): 2.51e-123: 400: 18.06		2AAA_HUMAN (1-589): 2.84e-166: 487: 100.00
+	Hs
		672185 MARK2_HUMAN (8-669): 2.09e-122: 399: 14.10		2AAA_HUMAN (1-589): 2.84e-166: 487: 100.00
+	Hs
		672006 AKT1_HUMAN (2-354): 2.35e-122: 388: 20.82		2AAA_HUMAN (1-589): 2.84e-166: 487: 100.00
+	Hs
		672414 AAPK1_HUMAN (8-555): 9.95e-123: 392: 16.85		2AAB_HUMAN (9-589): 7.23e-158: 466: 86.40
+	Hs
		131549 STK25_HUMAN (14-452): 5.34e-114: 364: 34.99		2AAA_HUMAN (1-589): 2.84e-166: 487: 100.00
+	Hs
		672332 STK26_HUMAN (16-414): 6.13e-111: 355: 34.05		2AAA_HUMAN (1-589): 2.84e-166: 487: 100.00
+	Hs
		672377 STK24_HUMAN (20-398): 2.05e-110: 355: 36.65		2AAA_HUMAN (1-589): 2.84e-166: 487: 100.00
+	Hs
		131550 STK25_HUMAN (14-452): 5.34e-114: 364: 34.99		2AAB_HUMAN (9-589): 7.23e-158: 466: 86.40
+	Hs
		672483 STK26_HUMAN (16-414): 6.13e-111: 355: 34.05		2AAB_HUMAN (9-589): 7.23e-158: 466: 86.40
+	Hs
		672501 STK24_HUMAN (20-398): 2.05e-110: 355: 36.65		2AAB_HUMAN (9-589): 7.23e-158: 466: 86.40
+	Dm
		734198 HIPPO_DROME (14-316): 8.94e-108: 356: 40.79		2AAA_DROME (1-578): 2.84e-146: 436: 75.35
+	Hs
		148220 CTRO_HUMAN (14-616): 3.12e-117: 405: 22.33		SF3B1_HUMAN (12-589): 2.5e-28: 122: 13.20
+	Hs
		1273247 SIK3_HUMAN (7-915): 2.5e-127: 426: 15.13		TNPO1_HUMAN (24-483): 2.91e-20: 97.2: 15.05
+	Hs
		149128 CTRO_HUMAN (14-616): 3.12e-117: 405: 22.33		GCN1_HUMAN (28-589): 5.37e-25: 112: 16.01