HitPredict

Interactor 1: M4K4 (O95819)
Mitogen-activated protein kinase kinase kinase kinase 4 (EC 2.7.11.1) (HPK/GCK-like kinase HGK) (MAPK/ERK kinase kinase kinase 4) (MEK kinase kinase 4) (MEKKK 4) (Nck-interacting kinase)
Gene: MAP4K4

Interactor 2: PP1G (P36873)
Serine/threonine-protein phosphatase PP1-gamma catalytic subunit (PP-1G) (EC 3.1.3.16) (Protein phosphatase 1C catalytic subunit)
Gene: PPP1CC

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.43	0.60	0.508

Property	Details
Experiments	21382349
Identification Methods	two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0000165 - MAPK cascade GO:0005737 - cytoplasm GO:0005925 - focal adhesion
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 1239 Hs			1 323
+	Hs
		324770 M4K4_HUMAN (1-1239): 0: 1227: 100.00		PP1A_HUMAN (1-323): 0: 579: 90.66
+	Hs
		324775 M4K4_HUMAN (1-1239): 0: 1227: 100.00		PP2AB_HUMAN (8-312): 2.02e-178: 497: 46.73
+	Hs
		324785 M4K4_HUMAN (1-1239): 0: 1227: 100.00		PP2AA_HUMAN (8-312): 5.65e-178: 496: 46.08
+	Mm
		926532 TNIK_MOUSE (1-1239): 0: 1120: 62.43		PP1G_MOUSE (1-323): 0: 602: 99.69
+	Mm
		906234 TNIK_MOUSE (1-1239): 0: 1120: 62.43		PP1A_MOUSE (1-323): 0: 579: 90.36
+	Hs
		91409 E7EN19_HUMAN (1-1239): 0: 1171: 91.79		PP2AB_HUMAN (8-312): 2.02e-178: 497: 46.73
+	Mm
		915321 TNIK_MOUSE (1-1239): 0: 1120: 62.43		PP2AB_MOUSE (8-312): 2.02e-178: 497: 46.73
+	Hs
		148470 CTRO_HUMAN (14-616): 3.12e-117: 405: 22.33		PP1G_HUMAN (1-323): 0: 603: 100.00
+	Hs
		681698 AKT1_HUMAN (2-354): 2.35e-122: 388: 20.82		PP1G_HUMAN (1-323): 0: 603: 100.00
+	Hs
		148613 CTRO_HUMAN (14-616): 3.12e-117: 405: 22.33		PP1A_HUMAN (1-323): 0: 579: 90.66
+	Mm
		682103 AKT1_MOUSE (2-354): 1.36e-122: 389: 20.82		PP1A_MOUSE (1-323): 0: 579: 90.36
+	Hs
		681767 AKT1_HUMAN (2-354): 2.35e-122: 388: 20.82		PP1A_HUMAN (1-323): 0: 579: 90.66
+	Hs
		148614 CTRO_HUMAN (14-616): 3.12e-117: 405: 22.33		PP1B_HUMAN (1-322): 0: 560: 86.89
+	Hs
		681768 AKT1_HUMAN (2-354): 2.35e-122: 388: 20.82		PP1B_HUMAN (1-322): 0: 560: 86.89
+	Hs
		915308 MAST1_HUMAN (3-947): 3.96e-126: 426: 14.68		PP2AB_HUMAN (8-312): 2.02e-178: 497: 46.73
+	Hs
		931047 MAST1_HUMAN (3-947): 3.96e-126: 426: 14.68		PP2AA_HUMAN (8-312): 5.65e-178: 496: 46.08
+	Hs
		647866 MARK3_HUMAN (16-665): 4.1e-127: 411: 16.23		PP2AB_HUMAN (8-312): 2.02e-178: 497: 46.73
+	Hs
		915261 MARK4_HUMAN (16-693): 2.51e-123: 400: 18.06		PP2AB_HUMAN (8-312): 2.02e-178: 497: 46.73
+	Hs
		682162 AKT2_HUMAN (2-354): 7.38e-124: 393: 21.98		PP2AA_HUMAN (8-312): 5.65e-178: 496: 46.08
+	Hs
		930826 AAPK1_HUMAN (8-555): 9.95e-123: 392: 16.85		PP2AA_HUMAN (8-312): 5.65e-178: 496: 46.08