HitPredict

Interactor 1: M4K4 (O95819)
Mitogen-activated protein kinase kinase kinase kinase 4 (EC 2.7.11.1) (HPK/GCK-like kinase HGK) (MAPK/ERK kinase kinase kinase 4) (MEK kinase kinase 4) (MEKKK 4) (Nck-interacting kinase)
Gene: MAP4K4

Interactor 2: 1433E (P62258)
14-3-3 protein epsilon (14-3-3E)
Gene: YWHAE

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.38	0.60	0.479

Property	Details
Experiments	36931259
Identification Methods	cross-linking study
Interaction Type	physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0000165 - MAPK cascade GO:0005737 - cytoplasm GO:0005925 - focal adhesion GO:0035556 - intracellular signal transduction
Homologous interactions	18

Homologous interactions

Color coding for hit scores

	1 1239 Hs			1 255
+	Hs
		909810 MINK1_HUMAN (1-1239): 0: 1120: 59.29		1433E_HUMAN (1-255): 4.87e-154: 430: 100.00
+	Hs
		910182 MAST1_HUMAN (3-947): 3.96e-126: 426: 14.68		1433E_HUMAN (1-255): 4.87e-154: 430: 100.00
+	Hs
		910187 SIK3_HUMAN (7-915): 2.5e-127: 426: 15.13		1433E_HUMAN (1-255): 4.87e-154: 430: 100.00
+	Hs
		909932 NEK1_HUMAN (23-917): 5.54e-125: 418: 17.52		1433E_HUMAN (1-255): 4.87e-154: 430: 100.00
+	Xl
		223344 Q804T2_XENLA (16-680): 2.52e-131: 421: 16.22		O57468_XENLA (1-255): 1.7e-152: 426: 96.47
+	Mm
		910231 MARK3_MOUSE (16-680): 4.82e-129: 416: 15.39		1433E_MOUSE (1-255): 4.87e-154: 430: 100.00
+	Hs
		647862 MARK3_HUMAN (16-665): 4.1e-127: 411: 16.23		1433E_HUMAN (1-255): 4.87e-154: 430: 100.00
+	Xl
		223345 Q8QGV3_XENLA (10-665): 2.84e-127: 412: 15.74		O57468_XENLA (1-255): 1.7e-152: 426: 96.47
+	Mm
		910234 MARK2_MOUSE (8-680): 1.5e-124: 405: 14.05		1433E_MOUSE (1-255): 4.87e-154: 430: 100.00
+	Hs
		909916 MARK4_HUMAN (16-693): 2.51e-123: 400: 18.06		1433E_HUMAN (1-255): 4.87e-154: 430: 100.00
+	Hs
		909730 MARK2_HUMAN (8-669): 2.09e-122: 399: 14.10		1433E_HUMAN (1-255): 4.87e-154: 430: 100.00
+	Mm
		910308 MARK4_MOUSE (16-693): 1.14e-122: 399: 17.92		1433E_MOUSE (1-255): 4.87e-154: 430: 100.00
+	Hs
		909507 AAPK1_HUMAN (8-555): 9.95e-123: 392: 16.85		1433E_HUMAN (1-255): 4.87e-154: 430: 100.00
+	Hs
		681770 AKT1_HUMAN (2-354): 2.35e-122: 388: 20.82		1433E_HUMAN (1-255): 4.87e-154: 430: 100.00
+	Mm
		910248 BRSK1_MOUSE (22-753): 4.03e-118: 387: 16.95		1433E_MOUSE (1-255): 4.87e-154: 430: 100.00
+	Sc
		630981 KCC4_YEAST (22-923): 2.08e-131: 430: 16.02		BMH1_YEAST (2-243): 2.43e-134: 381: 75.72
+	Hs
		909512 STK3_HUMAN (4-499): 3.17e-119: 381: 30.60		1433E_HUMAN (1-255): 4.87e-154: 430: 100.00
+	Hs
		909502 STK4_HUMAN (4-517): 8.55e-119: 379: 31.92		1433E_HUMAN (1-255): 4.87e-154: 430: 100.00