HitPredict

Interactor 1: M4K4 (O95819)
Mitogen-activated protein kinase kinase kinase kinase 4 (EC 2.7.11.1) (HPK/GCK-like kinase HGK) (MAPK/ERK kinase kinase kinase 4) (MEK kinase kinase 4) (MEKKK 4) (Nck-interacting kinase)
Gene: MAP4K4

Interactor 2: GRB2 (P62993)
Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2)
Gene: GRB2

Species: Homo sapiens

Source: biogrid

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.56	0.60	0.584

Property	Details
Experiments	32640226, 36736316
Identification Methods	affinity chromatography technology
Interaction Type	physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm GO:0043408 - regulation of MAPK cascade
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 1239 Hs			1 217
+	Hs
		324717 M4K4_HUMAN (1-1239): 0: 1227: 100.00		LCK_HUMAN (3-202): 3.69e-37: 137: 28.91
+	Hs
		924703 MINK1_HUMAN (1-1239): 0: 1120: 59.29		GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00
+	Hs
		324734 M4K4_HUMAN (1-1239): 0: 1227: 100.00		NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14
+	Hs
		324719 M4K4_HUMAN (1-1239): 0: 1227: 100.00		LYN_HUMAN (129-215): 1.44e-11: 65: 22.47
+	Hs
		411790 MINK1_HUMAN (1-1239): 0: 1120: 59.29		LCK_HUMAN (3-202): 3.69e-37: 137: 28.91
+	Mm
		956970 M4K4_MOUSE (1-1239): 0: 1142: 88.17		NCK1_MOUSE (5-145): 8.99e-23: 96.3: 33.14
+	Hs
		557740 MINK1_HUMAN (1-1239): 0: 1120: 59.29		NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14
+	Hs
		557820 TNIK_HUMAN (1-1239): 0: 1101: 60.56		NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14
+	Hs
		433891 MINK1_HUMAN (1-1239): 0: 1120: 59.29		LYN_HUMAN (129-215): 1.44e-11: 65: 22.47
+	Hs
		434067 TNIK_HUMAN (1-1239): 0: 1101: 60.56		LYN_HUMAN (129-215): 1.44e-11: 65: 22.47
+	Dm
		993188 Q7KV89_DROME (3-813): 4.51e-117: 393: 40.32		GRAP_DROME (1-215): 5.1e-62: 194: 62.79
+	Hs
		411606 MARK3_HUMAN (16-665): 4.1e-127: 411: 16.23		LCK_HUMAN (3-202): 3.69e-37: 137: 28.91
+	Hs
		411778 MARK2_HUMAN (8-669): 2.09e-122: 399: 14.10		LCK_HUMAN (3-202): 3.69e-37: 137: 28.91
+	Hs
		782836 MAST1_HUMAN (3-947): 3.96e-126: 426: 14.68		CSK_HUMAN (2-139): 3.92e-24: 100: 36.24
+	Hs
		433589 MARK3_HUMAN (16-665): 4.1e-127: 411: 16.23		LYN_HUMAN (129-215): 1.44e-11: 65: 22.47
+	Hs
		433949 MARK4_HUMAN (16-693): 2.51e-123: 400: 18.06		LYN_HUMAN (129-215): 1.44e-11: 65: 22.47
+	Hs
		412167 AAPK1_HUMAN (8-555): 9.95e-123: 392: 16.85		FYN_HUMAN (161-215): 4.44e-14: 72.3: 31.58
+	Hs
		433850 MARK2_HUMAN (8-669): 2.09e-122: 399: 14.10		LYN_HUMAN (129-215): 1.44e-11: 65: 22.47
+	Hs
		433609 AKT2_HUMAN (2-354): 7.38e-124: 393: 21.98		LYN_HUMAN (129-215): 1.44e-11: 65: 22.47
+	Mm
		403418 AKT1_MOUSE (2-354): 1.36e-122: 389: 20.82		SRC_MOUSE (161-215): 3.76e-11: 63.8: 31.58