HitPredict

Interactor 1: M4K4 (O95819)
Mitogen-activated protein kinase kinase kinase kinase 4 (EC 2.7.11.1) (HPK/GCK-like kinase HGK) (MAPK/ERK kinase kinase kinase 4) (MEK kinase kinase 4) (MEKKK 4) (Nck-interacting kinase)
Gene: MAP4K4

Interactor 2: RAC1 (P63000)
Ras-related C3 botulinum toxin substrate 1 (EC 3.6.5.2) (Cell migration-inducing gene 5 protein) (Ras-like protein TC25) (p21-Rac1)
Gene: RAC1

Species: Homo sapiens

Source: biogrid

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.38	1.00	0.618

Property	Details
Experiments	31871319
Identification Methods	affinity chromatography technology
Interaction Type	physical association

Property	Details
Interacting Pfam domains	Pkinase~Ras
Common GO terms	GO:0005737 - cytoplasm GO:0005925 - focal adhesion GO:0035556 - intracellular signal transduction GO:0051894 - positive regulation of focal adhesion assembly
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 1239 Hs			1 192
+	Hs
		324732 M4K4_HUMAN (1-1239): 0: 1227: 100.00		RAC2_HUMAN (1-192): 1.2e-82: 244: 92.19
+	Hs
		324877 M4K4_HUMAN (1-1239): 0: 1227: 100.00		RHOJ_HUMAN (2-189): 1.38e-80: 240: 60.64
+	Hs
		324768 M4K4_HUMAN (1-1239): 0: 1227: 100.00		RAC3_HUMAN (1-191): 2.72e-79: 236: 93.19
+	Hs
		324789 M4K4_HUMAN (1-1239): 0: 1227: 100.00		RHOG_HUMAN (1-192): 3.1e-79: 236: 71.35
+	Hs
		925434 MINK1_HUMAN (1-1239): 0: 1120: 59.29		RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00
+	Mm
		925783 TNIK_MOUSE (1-1239): 0: 1120: 62.43		RAC1_MOUSE (1-192): 3.81e-86: 253: 100.00
+	Hs
		564611 MINK1_HUMAN (1-1239): 0: 1120: 59.29		RHOQ_HUMAN (4-187): 4.46e-85: 251: 64.67
+	Hs
		545493 MINK1_HUMAN (1-1239): 0: 1120: 59.29		RAC2_HUMAN (1-192): 1.2e-82: 244: 92.19
+	Hs
		1233542 MINK1_HUMAN (1-1239): 0: 1120: 59.29		RHOJ_HUMAN (2-189): 1.38e-80: 240: 60.64
+	Hs
		892483 MINK1_HUMAN (1-1239): 0: 1120: 59.29		RAC3_HUMAN (1-191): 2.72e-79: 236: 93.19
+	Hs
		925356 MRCKG_HUMAN (13-974): 7.6e-138: 459: 17.29		RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00
+	Hs
		564571 MRCKG_HUMAN (13-974): 7.6e-138: 459: 17.29		RHOQ_HUMAN (4-187): 4.46e-85: 251: 64.67
+	Hs
		894128 MRCKG_HUMAN (13-974): 7.6e-138: 459: 17.29		CDC42_HUMAN (1-192): 1.4e-78: 234: 70.31
+	Dm
		776947 GEK_DROME (15-983): 3.23e-136: 456: 13.20		CDC42_DROME (1-192): 2.41e-77: 231: 70.83
+	Hs
		564418 MARK3_HUMAN (16-665): 4.1e-127: 411: 16.23		RHOQ_HUMAN (4-187): 4.46e-85: 251: 64.67
+	Hs
		545213 MARK3_HUMAN (16-665): 4.1e-127: 411: 16.23		RAC2_HUMAN (1-192): 1.2e-82: 244: 92.19
+	Hs
		647855 MARK3_HUMAN (16-665): 4.1e-127: 411: 16.23		RAC3_HUMAN (1-191): 2.72e-79: 236: 93.19
+	Hs
		647856 MARK3_HUMAN (16-665): 4.1e-127: 411: 16.23		CDC42_HUMAN (1-192): 1.4e-78: 234: 70.31
+	Hs
		647858 MARK3_HUMAN (16-665): 4.1e-127: 411: 16.23		RHOA_HUMAN (5-192): 3.5e-77: 230: 57.98
+	Hs
		436165 MARK3_HUMAN (16-665): 4.1e-127: 411: 16.23		RHOC_HUMAN (5-192): 8.68e-75: 224: 57.45