HitPredict

Interactor 1: EGFR (P00533)
Epidermal growth factor receptor (EC 2.7.10.1) (Proto-oncogene c-ErbB-1) (Receptor tyrosine-protein kinase erbB-1)
Gene: EGFR

Interactor 2: 1433T (P27348)
14-3-3 protein theta (14-3-3 protein T-cell) (14-3-3 protein tau) (Protein HS1)
Gene: YWHAQ

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.88	1.00	0.941

Property	Details
Experiments	25402006, 24658140, 20029029, unassigned2173, 15657067, 24189400
Identification Methods	two hybrid, protein complementation assay, anti tag coimmunoprecipitation, ubiquitin reconstruction, anti bait coimmunoprecipitation, tandem affinity purification
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	14-3-3~PK_Tyr_Ser-Thr
Common GO terms	GO:0005737 - cytoplasm GO:0005925 - focal adhesion GO:0007165 - signal transduction GO:0016020 - membrane GO:0032991 - protein-containing complex
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 1210 Hs			1 245
+	Hs
		338232 EGFR_HUMAN (1-1210): 0: 1241: 100.00		1433E_HUMAN (2-243): 1.31e-144: 406: 63.53
+	Hs
		338257 EGFR_HUMAN (1-1210): 0: 1241: 100.00		1433Z_HUMAN (1-245): 1.75e-141: 397: 79.59
+	Hs
		338008 EGFR_HUMAN (1-1210): 0: 1241: 100.00		1433B_HUMAN (1-242): 5.01e-139: 391: 81.82
+	Hs
		338009 EGFR_HUMAN (1-1210): 0: 1241: 100.00		1433S_HUMAN (1-236): 9.86e-108: 312: 71.85
+	Hs
		909589 ERBB4_HUMAN (1-1199): 0: 1137: 49.46		1433E_HUMAN (2-243): 1.31e-144: 406: 63.53
+	Hs
		383724 ERBB2_HUMAN (5-1200): 0: 1111: 48.49		1433E_HUMAN (2-243): 1.31e-144: 406: 63.53
+	Hs
		383624 ERBB2_HUMAN (5-1200): 0: 1111: 48.49		1433T_HUMAN (1-245): 9.47e-142: 398: 100.00
+	Hs
		383732 ERBB2_HUMAN (5-1200): 0: 1111: 48.49		1433Z_HUMAN (1-245): 1.75e-141: 397: 79.59
+	Hs
		601877 ERBB3_HUMAN (1-1210): 0: 1080: 40.95		1433Z_HUMAN (1-245): 1.75e-141: 397: 79.59
+	Hs
		684051 ERBB4_HUMAN (1-1199): 0: 1137: 49.46		1433S_HUMAN (1-236): 9.86e-108: 312: 71.85
+	Hs
		383641 ERBB2_HUMAN (5-1200): 0: 1111: 48.49		1433S_HUMAN (1-236): 9.86e-108: 312: 71.85
+	At
		788498 TMK1_ARATH (345-984): 1.18e-139: 449: 15.52		14333_ARATH (3-243): 8e-143: 401: 61.63
+	At
		965337 TMK2_ARATH (337-961): 4.88e-134: 433: 16.71		14332_ARATH (3-243): 8.65e-144: 404: 61.22
+	Hs
		411315 INSR_HUMAN (451-1026): 9.86e-109: 374: 20.87		1433B_HUMAN (1-242): 5.01e-139: 391: 81.82
+	Hs
		411316 INSR_HUMAN (451-1026): 9.86e-109: 374: 20.87		1433S_HUMAN (1-236): 9.86e-108: 312: 71.85
+	Gg
		94492 KSYK_CHICK (718-961): 1.71e-55: 206: 41.30		1433E_CHICK (2-243): 1.31e-144: 406: 63.53
+	Hs
		926964 ACK1_HUMAN (691-967): 4.54e-56: 214: 39.58		1433Z_HUMAN (1-245): 1.75e-141: 397: 79.59
+	Gg
		94476 KSYK_CHICK (718-961): 1.71e-55: 206: 41.30		1433Z_CHICK (1-245): 7.94e-141: 396: 79.18
+	Hs
		904484 ACK1_HUMAN (691-967): 4.54e-56: 214: 39.58		1433G_HUMAN (1-242): 5.48e-105: 305: 70.33
+	Hs
		904472 FAK1_HUMAN (716-991): 1.58e-53: 206: 37.68		1433G_HUMAN (1-242): 5.48e-105: 305: 70.33