HitPredict

Interactor 1: RASN (P01111)
GTPase NRas (EC 3.6.5.2) (Transforming protein N-Ras)
Gene: NRAS

Interactor 2: RAF1 (P04049)
RAF proto-oncogene serine/threonine-protein kinase (EC 2.7.11.1) (Proto-oncogene c-RAF) (cRaf) (Raf-1)
Gene: RAF1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.96	1.00	0.980

Property	Details
Experiments	30639242, 30194290, 32296183, 31980649, 10882715, 7766599, 36244648, 18641128, 20080631, 21478863, 8876196, unassigned1304, unassigned2173, 34591642
Identification Methods	proximity labelling technology, affinity chromatography technology, two hybrid, pull down, fluorescent resonance energy transfer, anti tag coimmunoprecipitation, experimental interaction detection, two hybrid prey pooling approach, two hybrid array, validated two hybrid
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	C1_1~Ras, Pkinase~Ras, RBD~Ras
Common GO terms	GO:0000165 - MAPK cascade GO:0005794 - Golgi apparatus GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0007265 - Ras protein signal transduction
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 189 Hs			1 648
+	Hs
		349785 RASK_HUMAN (16-189): 7.56e-85: 249: 85.71		RAF1_HUMAN (1-648): 0: 652: 100.00
+	Hs
		373973 RAB8A_HUMAN (4-185): 2.24e-84: 249: 36.02		RAF1_HUMAN (1-648): 0: 652: 100.00
+	Rn
		442943 RASK_RAT (1-189): 3.25e-85: 251: 87.90		RAF1_RAT (1-648): 0: 648: 98.30
+	Mm
		699624 RASK_MOUSE (1-189): 3.25e-85: 251: 87.90		RAF1_MOUSE (1-648): 0: 647: 97.69
+	Rn
		507685 RASH_RAT (1-188): 8.6e-78: 232: 85.64		RAF1_RAT (1-648): 0: 648: 98.30
+	Hs
		349847 RASK_HUMAN (16-189): 7.56e-85: 249: 85.71		BRAF_HUMAN (39-647): 1.29e-170: 507: 55.88
+	Dm
		442988 RAS1_DROME (1-170): 2.87e-90: 263: 83.53		KRAF1_DROME (45-647): 6.35e-165: 491: 44.98
+	Hs
		349820 RASK_HUMAN (16-189): 7.56e-85: 249: 85.71		ARAF_HUMAN (45-646): 4.63e-169: 497: 59.28
+	Mm
		384059 RASK_MOUSE (1-189): 3.25e-85: 251: 87.90		ARAF_MOUSE (45-646): 4.46e-168: 495: 58.65
+	Hs
		348493 RASN_HUMAN (1-189): 9.9e-80: 236: 100.00		BRAF_HUMAN (39-647): 1.29e-170: 507: 55.88
+	Hs
		350452 RASK_HUMAN (16-189): 7.56e-85: 249: 85.71		Q96II5_HUMAN (45-646): 9.01e-168: 494: 58.82
+	Hs
		348480 RASN_HUMAN (1-189): 9.9e-80: 236: 100.00		ARAF_HUMAN (45-646): 4.63e-169: 497: 59.28
+	Hs
		348865 RASN_HUMAN (1-189): 9.9e-80: 236: 100.00		Q96II5_HUMAN (45-646): 9.01e-168: 494: 58.82
+	Ce
		612364 LET60_CAEEL (1-181): 1.39e-88: 259: 76.80		KRAF1_CAEEL (50-627): 1.76e-151: 459: 35.95
+	Hs
		349836 RASK_HUMAN (16-189): 7.56e-85: 249: 85.71		SRC_HUMAN (331-607): 3.01e-106: 333: 33.45
+	Hs
		349806 RASK_HUMAN (16-189): 7.56e-85: 249: 85.71		YES_HUMAN (329-607): 1.22e-105: 332: 33.92
+	Hs
		433358 RAB35_HUMAN (4-171): 6.96e-84: 247: 34.71		YES_HUMAN (329-607): 1.22e-105: 332: 33.92
+	Hs
		348488 RASN_HUMAN (1-189): 9.9e-80: 236: 100.00		SRC_HUMAN (331-607): 3.01e-106: 333: 33.45
+	Hs
		348473 RASN_HUMAN (1-189): 9.9e-80: 236: 100.00		YES_HUMAN (329-607): 1.22e-105: 332: 33.92
+	Mm
		699627 RASK_MOUSE (1-189): 3.25e-85: 251: 87.90		Q9JJU6_MOUSE (43-297): 2.38e-55: 194: 42.38