HitPredict

Interactor 1: RASN (P01111)
GTPase NRas (EC 3.6.5.2) (Transforming protein N-Ras)
Gene: NRAS

Interactor 2: ARAF (P10398)
Serine/threonine-protein kinase A-Raf (EC 2.7.11.1) (Proto-oncogene A-Raf) (Proto-oncogene A-Raf-1) (Proto-oncogene Pks)
Gene: ARAF

Species: Homo sapiens

Source: mint

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.75	1.00	0.870

Property	Details
Experiments	30194290, 29777862, 32296183, 34591642
Identification Methods	proximity labelling technology, affinity chromatography technology, two hybrid, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	C1_1~Ras, RBD~Ras
Common GO terms	GO:0000165 - MAPK cascade GO:0005829 - cytosol GO:0007265 - Ras protein signal transduction
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 189 Hs			1 606
+	Hs
		349820 RASK_HUMAN (16-189): 7.56e-85: 249: 85.71		ARAF_HUMAN (1-606): 0: 579: 100.00
+	Hs
		350452 RASK_HUMAN (16-189): 7.56e-85: 249: 85.71		Q96II5_HUMAN (1-606): 0: 573: 99.51
+	Mm
		384059 RASK_MOUSE (1-189): 3.25e-85: 251: 87.90		ARAF_MOUSE (1-606): 0: 563: 94.88
+	Hs
		348865 RASN_HUMAN (1-189): 9.9e-80: 236: 100.00		Q96II5_HUMAN (1-606): 0: 573: 99.51
+	Hs
		349847 RASK_HUMAN (16-189): 7.56e-85: 249: 85.71		BRAF_HUMAN (3-604): 0: 546: 56.68
+	Hs
		349785 RASK_HUMAN (16-189): 7.56e-85: 249: 85.71		RAF1_HUMAN (13-606): 0: 534: 60.53
+	Hs
		373973 RAB8A_HUMAN (4-185): 2.24e-84: 249: 36.02		RAF1_HUMAN (13-606): 0: 534: 60.53
+	Hs
		348493 RASN_HUMAN (1-189): 9.9e-80: 236: 100.00		BRAF_HUMAN (3-604): 0: 546: 56.68
+	Rn
		442943 RASK_RAT (1-189): 3.25e-85: 251: 87.90		RAF1_RAT (13-606): 0: 529: 60.20
+	Mm
		699624 RASK_MOUSE (1-189): 3.25e-85: 251: 87.90		RAF1_MOUSE (13-606): 0: 527: 59.37
+	Hs
		348461 RASN_HUMAN (1-189): 9.9e-80: 236: 100.00		RAF1_HUMAN (13-606): 0: 534: 60.53
+	Rn
		507685 RASH_RAT (1-188): 8.6e-78: 232: 85.64		RAF1_RAT (13-606): 0: 529: 60.20
+	Dm
		442988 RAS1_DROME (1-170): 2.87e-90: 263: 83.53		KRAF1_DROME (5-605): 2.05e-167: 496: 42.18
+	Ce
		612364 LET60_CAEEL (1-181): 1.39e-88: 259: 76.80		KRAF1_CAEEL (6-606): 3.19e-151: 457: 33.94
+	Hs
		349806 RASK_HUMAN (16-189): 7.56e-85: 249: 85.71		YES_HUMAN (289-568): 3.31e-104: 327: 31.34
+	Hs
		349836 RASK_HUMAN (16-189): 7.56e-85: 249: 85.71		SRC_HUMAN (282-568): 1.92e-104: 327: 30.24
+	Hs
		433358 RAB35_HUMAN (4-171): 6.96e-84: 247: 34.71		YES_HUMAN (289-568): 3.31e-104: 327: 31.34
+	Hs
		348473 RASN_HUMAN (1-189): 9.9e-80: 236: 100.00		YES_HUMAN (289-568): 3.31e-104: 327: 31.34
+	Hs
		348488 RASN_HUMAN (1-189): 9.9e-80: 236: 100.00		SRC_HUMAN (282-568): 1.92e-104: 327: 30.24
+	Mm
		699627 RASK_MOUSE (1-189): 3.25e-85: 251: 87.90		Q9JJU6_MOUSE (3-270): 1.28e-56: 197: 43.82