HitPredict

Interactor 1: RASN (P01111)
GTPase NRas (EC 3.6.5.2) (Transforming protein N-Ras)
Gene: NRAS

Interactor 2: P85A (P27986)
Phosphatidylinositol 3-kinase regulatory subunit alpha (PI3-kinase regulatory subunit alpha) (PI3K regulatory subunit alpha) (PtdIns-3-kinase regulatory subunit alpha) (Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha) (PI3-kinase subunit p85-alpha) (PtdIns-3-kinase regulatory subunit p85-alpha)
Gene: PIK3R1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.44	1.00	0.665

Property	Details
Experiments	32814053
Identification Methods	two hybrid array, two hybrid pooling approach, validated two hybrid
Interaction Type	physical association

Property	Details
Interacting Pfam domains	Ras~RhoGAP
Common GO terms	GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0016020 - membrane
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 189 Hs			1 724
+	Hs
		349897 RASK_HUMAN (16-189): 7.56e-85: 249: 85.71		P85A_HUMAN (1-724): 0: 596: 100.00
+	Hs
		655376 RAB35_HUMAN (4-171): 6.96e-84: 247: 34.71		P85A_HUMAN (1-724): 0: 596: 100.00
+	Hs
		349141 RASH_HUMAN (1-188): 8.6e-78: 232: 85.64		P85A_HUMAN (1-724): 0: 596: 100.00
+	Mm
		643094 RASH_MOUSE (1-188): 8.6e-78: 232: 85.64		P85A_MOUSE (1-724): 0: 592: 95.99
+	Hs
		129153 RASK_HUMAN (16-189): 7.56e-85: 249: 85.71		P85B_HUMAN (4-719): 6.38e-162: 486: 57.24
+	Hs
		129294 RAB35_HUMAN (4-171): 6.96e-84: 247: 34.71		P85B_HUMAN (4-719): 6.38e-162: 486: 57.24
+	Hs
		129261 RAB2A_HUMAN (4-185): 1.53e-73: 222: 31.52		P85B_HUMAN (4-719): 6.38e-162: 486: 57.24
+	Hs
		1070328 RAB35_HUMAN (4-171): 6.96e-84: 247: 34.71		P55G_HUMAN (271-723): 1.4e-104: 329: 72.31
+	Hs
		914255 RB11A_HUMAN (4-184): 1.5e-74: 224: 33.88		P55G_HUMAN (271-723): 1.4e-104: 329: 72.31
+	Hs
		64637 RASK_HUMAN (16-189): 7.56e-85: 249: 85.71		RHG32_HUMAN (14-613): 1.13e-61: 227: 13.99
+	Hs
		64708 RAB35_HUMAN (4-171): 6.96e-84: 247: 34.71		RHG32_HUMAN (14-613): 1.13e-61: 227: 13.99
+	Hs
		350234 RASK_HUMAN (16-189): 7.56e-85: 249: 85.71		RHG21_HUMAN (78-531): 1.19e-59: 221: 13.46
+	Hs
		1070234 RAB35_HUMAN (4-171): 6.96e-84: 247: 34.71		RHG21_HUMAN (78-531): 1.19e-59: 221: 13.46
+	Hs
		349384 RASH_HUMAN (1-188): 8.6e-78: 232: 85.64		RHG21_HUMAN (78-531): 1.19e-59: 221: 13.46
+	Hs
		253300 RASK_HUMAN (16-189): 7.56e-85: 249: 85.71		SRGP2_HUMAN (118-668): 3.41e-54: 203: 13.78
+	Hs
		349806 RASK_HUMAN (16-189): 7.56e-85: 249: 85.71		YES_HUMAN (568-724): 2.15e-31: 131: 20.61
+	Hs
		433358 RAB35_HUMAN (4-171): 6.96e-84: 247: 34.71		YES_HUMAN (568-724): 2.15e-31: 131: 20.61
+	Hs
		348473 RASN_HUMAN (1-189): 9.9e-80: 236: 100.00		YES_HUMAN (568-724): 2.15e-31: 131: 20.61
+	Hs
		349069 RASH_HUMAN (1-188): 8.6e-78: 232: 85.64		YES_HUMAN (568-724): 2.15e-31: 131: 20.61
+	Hs
		433312 RB11A_HUMAN (4-184): 1.5e-74: 224: 33.88		YES_HUMAN (568-724): 2.15e-31: 131: 20.61