HitPredict

Interactor 1: RAS2 (P01120)
Ras-like protein 2 (EC 3.6.5.2)
Gene: RAS2

Interactor 2: LTE1 (P07866)
Guanine nucleotide exchange factor LTE1 (Low temperature essential protein 1)
Gene: LTE1

Species: Saccharomyces cerevisiae S288C

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.81	1.00	0.902

Property	Details
Experiments	12782684, 19948498, 21149565, 21670215
Identification Methods	two hybrid, affinity chromatography technology, pull down, anti tag coimmunoprecipitation
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	Ras~RasGEF, Ras~RasGEF_N
Common GO terms	GO:0005886 - plasma membrane GO:0007265 - Ras protein signal transduction
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 322 Sc			1 1435
+	Sc
		350756 RAS1_YEAST (1-321): 1.11e-90: 274: 57.72		LTE1_YEAST (1-1435): 0: 2228: 100.00
+	Sc
		431335 RSR1_YEAST (8-277): 1.17e-84: 258: 37.00		LTE1_YEAST (1-1435): 0: 2228: 100.00
+	Hs
		919681 RAP1A_HUMAN (8-171): 1.27e-61: 196: 59.39		RGL1_HUMAN (1027-1434): 5.22e-103: 348: 16.56
+	Dm
		442976 RAP1_DROME (8-171): 1.01e-84: 254: 59.15		Q9VMF3_DROME (1089-1432): 2.58e-78: 288: 14.84
+	Hs
		897964 RAP1B_HUMAN (8-171): 2.27e-60: 192: 60.00		RGL1_HUMAN (1027-1434): 5.22e-103: 348: 16.56
+	Hs
		919653 RAP1A_HUMAN (8-171): 1.27e-61: 196: 59.39		RPGF6_HUMAN (1013-1418): 2.19e-87: 316: 16.89
+	Hs
		919687 RAP1A_HUMAN (8-171): 1.27e-61: 196: 59.39		RPGF2_HUMAN (1012-1427): 1.14e-86: 314: 16.59
+	Hs
		919625 RAP1A_HUMAN (8-171): 1.27e-61: 196: 59.39		RPGF1_HUMAN (1067-1431): 2.54e-81: 293: 16.58
+	Hs
		919656 RAP1A_HUMAN (8-171): 1.27e-61: 196: 59.39		RPGF4_HUMAN (1142-1434): 2.07e-78: 283: 18.42
+	Hs
		171541 RAP1B_HUMAN (8-171): 2.27e-60: 192: 60.00		RGL2_HUMAN (1016-1434): 6.67e-78: 277: 14.51
+	Hs
		919618 RAP1A_HUMAN (8-171): 1.27e-61: 196: 59.39		GNDS_HUMAN (1129-1434): 9.38e-76: 273: 18.05
+	Hs
		171543 RRAS2_HUMAN (6-172): 5.33e-59: 189: 58.68		RGL2_HUMAN (1016-1434): 6.67e-78: 277: 14.51
+	Hs
		897883 RAP1B_HUMAN (8-171): 2.27e-60: 192: 60.00		GNDS_HUMAN (1129-1434): 9.38e-76: 273: 18.05
+	Hs
		919657 RAP1A_HUMAN (8-171): 1.27e-61: 196: 59.39		RPGF5_HUMAN (1072-1434): 1.14e-75: 265: 19.19
+	Sc
		350755 RAS1_YEAST (1-321): 1.11e-90: 274: 57.72		CDC25_YEAST (1190-1403): 3.16e-44: 178: 25.00
+	Hs
		313171 RAP1A_HUMAN (8-171): 1.27e-61: 196: 59.39		RPGF3_HUMAN (1107-1435): 1.46e-68: 252: 14.29
+	Sc
		350781 RAS2_YEAST (1-322): 2.57e-88: 269: 100.00		CDC25_YEAST (1190-1403): 3.16e-44: 178: 25.00
+	Hs
		313169 RAP1B_HUMAN (8-171): 2.27e-60: 192: 60.00		RPGF3_HUMAN (1107-1435): 1.46e-68: 252: 14.29
+	Ce
		1089983 RAP1_CAEEL (8-188): 1.1e-84: 254: 54.70		Q19770_CAEEL (1194-1396): 2.86e-44: 173: 23.45
+	Hs
		919645 RAP1A_HUMAN (8-171): 1.27e-61: 196: 59.39		RGDSR_HUMAN (1184-1434): 1.07e-61: 221: 16.79