HitPredict

Interactor 1: RL25 (P04456)
Large ribosomal subunit protein uL23 (60S ribosomal protein L25) (RP16L) (YL25) (YP42')
Gene: RPL25

Interactor 2: SPB4 (P25808)
ATP-dependent rRNA helicase SPB4 (EC 3.6.4.13) (Suppressor of PAB1 protein 4)
Gene: SPB4

Species: Saccharomyces cerevisiae S288C

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.62	0.60	0.611

Property	Details
Experiments	16429126, 31062022
Identification Methods	affinity chromatography technology, tandem affinity purification
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0000027 - ribosomal large subunit assembly GO:0003723 - RNA binding GO:0030687 - preribosome, large subunit precursor
Homologous interactions	8

Homologous interactions

Color coding for hit scores

	1 142 Sc			1 606
+	Hs
		916406 RL23A_HUMAN (17-142): 2.68e-59: 182: 62.70		DDX18_HUMAN (6-467): 8.02e-159: 472: 29.61
+	Sc
		383328 RL25_YEAST (1-142): 9.11e-58: 177: 100.00		HAS1_YEAST (6-467): 1.01e-156: 460: 30.98
+	Sc
		382851 RL25_YEAST (1-142): 9.11e-58: 177: 100.00		DED1_YEAST (3-402): 8.68e-139: 418: 25.55
+	Sc
		382965 RL25_YEAST (1-142): 9.11e-58: 177: 100.00		DBP2_YEAST (2-402): 7.47e-126: 383: 25.43
+	Hs
		916274 RL23A_HUMAN (17-142): 2.68e-59: 182: 62.70		DDX55_HUMAN (6-594): 5.25e-122: 375: 34.05
+	Sc
		382945 RL25_YEAST (1-142): 9.11e-58: 177: 100.00		DBP4_YEAST (21-467): 7e-51: 189: 31.36
+	Hs
		916171 RL23A_HUMAN (17-142): 2.68e-59: 182: 62.70		DDX10_HUMAN (21-472): 5.23e-47: 179: 29.67
+	Sc
		383037 RL25_YEAST (1-142): 9.11e-58: 177: 100.00		DRS1_YEAST (21-392): 3.9e-47: 178: 31.75