HitPredict

Interactor 1: TOLC (P02930)
Outer membrane protein TolC (Multidrug efflux pump subunit TolC) (Outer membrane factor TolC)
Gene: TOLC

Interactor 2: ACRA (P0AE06)
Multidrug efflux pump subunit AcrA (AcrAB-TolC multidrug efflux pump subunit AcrA) (Acridine resistance protein A)
Gene: ACRA

Species: Escherichia coli K-12

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.69	1.00	0.834

The method score is based on:

Property	Details
Experiments	29176613, 19805313, 15576805
Identification Methods	affinity chromatography technology, surface plasmon resonance
Interaction Type	physical association, direct interaction

The annotation score is based on:

Property	Details
Interacting Pfam domains	CusB_dom_1~OEP, HlyD_D23~OEP
Common GO terms	GO:0009410 - response to xenobiotic stimulus GO:0009636 - response to toxic substance GO:0015125 - bile acid transmembrane transporter activity GO:0015721 - bile acid and bile salt transport GO:0030288 - outer membrane-bounded periplasmic space and 4 others more... GO:0046677 - response to antibiotic GO:0098567 - periplasmic side of plasma membrane GO:0140330 - xenobiotic detoxification by transmembrane export across the cell outer membrane GO:1990281 - efflux pump complex
Homologous interactions	4

Homologous interactions

Color coding for hit scores

	1	493
Ec

1	397

Service provided by Combinatics, Japan

Last updated: 11Aug2025