HitPredict

Interactor 1: A4 (P05067)
Amyloid-beta precursor protein (APP) (ABPP) (APPI) (Alzheimer disease amyloid A4 protein homolog) (Alzheimer disease amyloid protein) (Amyloid precursor protein) (Amyloid-beta (A4) precursor protein) (Amyloid-beta A4 protein) (Cerebral vascular amyloid peptide) (CVAP) (PreA4) (Protease nexin-II) (PN-II) [Cleaved into: N-APP; Soluble APP-alpha (S-APP-alpha); Soluble APP-beta (S-APP-beta); C99 (Beta-secretase C-terminal fragment) (Beta-CTF); Amyloid-beta protein 42 (Abeta42) (Beta-APP42); Amyloid-beta protein 40 (Abeta40) (Beta-APP40); C83 (Alpha-secretase C-terminal fragment) (Alpha-CTF); P3(42); P3(40); C80; Gamma-secretase C-terminal fragment 59 (Amyloid intracellular domain 59) (AICD-59) (AID(59)) (Gamma-CTF(59)); Gamma-secretase C-terminal fragment 57 (Amyloid intracellular domain 57) (AICD-57) (AID(57)) (Gamma-CTF(57)); Gamma-secretase C-terminal fragment 50 (Amyloid intracellular domain 50) (AICD-50) (AID(50)) (Gamma-CTF(50)); C31]
Gene: APP

Interactor 2: M3K5 (Q99683)
Mitogen-activated protein kinase kinase kinase 5 (EC 2.7.11.25) (Apoptosis signal-regulating kinase 1) (ASK-1) (MAPK/ERK kinase kinase 5) (MEK kinase 5) (MEKK 5)
Gene: MAP3K5

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.64	0.60	0.621

Property	Details
Experiments	17719230, 16049941
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation, anti bait coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0006468 - protein phosphorylation GO:0046330 - positive regulation of JNK cascade GO:0051402 - neuron apoptotic process
Homologous interactions	13

Homologous interactions

Color coding for hit scores

	1 770 Hs			1 1374
+	Mm
		192182 A4_MOUSE (1-770): 0: 1016: 96.62		M3K5_MOUSE (1-1372): 0: 1451: 94.13
+	Hs
		395949 A4_HUMAN (19-770): 0: 999: 100.00		MARK1_HUMAN (622-1374): 4.12e-143: 458: 15.27
+	Hs
		254432 A4_HUMAN (19-770): 0: 999: 100.00		ROCK2_HUMAN (668-1343): 7.3e-114: 392: 17.55
+	Hs
		394747 A4_HUMAN (19-770): 0: 999: 100.00		KS6A2_HUMAN (673-1074): 1.52e-117: 386: 20.91
+	Hs
		396020 A4_HUMAN (19-770): 0: 999: 100.00		KS6A6_HUMAN (673-1074): 2.65e-113: 375: 21.31
+	Hs
		160324 A4_HUMAN (19-770): 0: 999: 100.00		AURKA_HUMAN (551-947): 1.43e-115: 369: 22.36
+	Hs
		270362 A4_HUMAN (19-770): 0: 999: 100.00		KS6A5_HUMAN (673-1088): 5.06e-109: 365: 19.51
+	Hs
		394643 A4_HUMAN (19-770): 0: 999: 100.00		PAK1_HUMAN (686-959): 2.31e-97: 325: 31.02
+	Hs
		394647 A4_HUMAN (19-770): 0: 999: 100.00		PAK2_HUMAN (686-960): 1.26e-97: 325: 31.27
+	Hs
		131543 A4_HUMAN (19-770): 0: 999: 100.00		STK25_HUMAN (686-1026): 1.88e-97: 321: 31.29
+	Hs
		394194 A4_HUMAN (19-770): 0: 999: 100.00		AKT1_HUMAN (438-615): 7.18e-25: 112: 13.64
+	Hs
		394195 A4_HUMAN (19-770): 0: 999: 100.00		AKT2_HUMAN (438-620): 9.26e-25: 111: 13.24
+	Hs
		518051 CO6A3_HUMAN (251-348): 1.13e-23: 110: 28.57		NEK4_HUMAN (680-1303): 2.63e-113: 378: 19.70