HitPredict

Interactor 1: RS31 (P05759)
Ubiquitin-ribosomal protein eS31 fusion protein [Cleaved into: Ubiquitin; Small ribosomal subunit protein eS31 (40S ribosomal protein S31) (CEP76) (S37) (YS24)]
Gene: RPS31

Interactor 2: RSP5 (P39940)
E3 ubiquitin-protein ligase RSP5 (EC 2.3.2.26) (HECT-type E3 ubiquitin transferase RSP5) (Reverses SPT-phenotype protein 5)
Gene: RSP5

Species: Saccharomyces cerevisiae S288C

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.34	1.00	0.590

Property	Details
Experiments	18719252
Identification Methods	two hybrid
Interaction Type	physical association

Property	Details
Interacting Pfam domains	HECT~ubiquitin
Common GO terms	GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0016567 - protein ubiquitination
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 152 Sc			1 809
+	Sc
		477293 UBI4P_YEAST (1-76): 1.31e-32: 120: 100.00		RSP5_YEAST (1-809): 0: 825: 100.00
+	Sp
		145417 RS27B_SCHPO (1-152): 7.75e-54: 168: 86.18		PUB3_SCHPO (4-809): 0: 733: 54.05
+	Sp
		145416 RS27A_SCHPO (1-151): 3.91e-53: 166: 86.09		PUB3_SCHPO (4-809): 0: 733: 54.05
+	Hs
		924156 RS27A_HUMAN (1-151): 1.46e-67: 203: 82.78		WWP1_HUMAN (108-809): 0: 690: 40.33
+	Hs
		125347 RS27A_HUMAN (1-151): 1.46e-67: 203: 82.78		WWP2_HUMAN (100-809): 0: 684: 40.81
+	Sp
		145418 RL402_SCHPO (1-76): 2.96e-42: 138: 100.00		PUB3_SCHPO (4-809): 0: 733: 54.05
+	Rn
		1140970 UBC_RAT (1-135): 9.19e-34: 126: 57.78		Q5YB86_RAT (1-809): 0: 739: 36.17
+	Hs
		475140 UBB_HUMAN (1-76): 4.1e-32: 115: 96.05		ITCH_HUMAN (1-809): 0: 727: 34.62
+	Hs
		125112 UBB_HUMAN (1-76): 4.1e-32: 115: 96.05		WWP2_HUMAN (100-809): 0: 684: 40.81
+	Hs
		474953 UBB_HUMAN (1-76): 4.1e-32: 115: 96.05		NEDD4_HUMAN (102-807): 0: 668: 39.67
+	Hs
		475196 UBB_HUMAN (1-76): 4.1e-32: 115: 96.05		SMUF2_HUMAN (230-809): 0: 640: 42.86
+	Mm
		924239 RS27A_MOUSE (1-151): 5.26e-67: 202: 82.12		HUWE1_MOUSE (352-808): 7.81e-167: 538: 37.42
+	Sc
		408448 RS31_YEAST (1-152): 3.66e-57: 177: 100.00		TOM1_YEAST (370-808): 5.87e-157: 509: 37.36
+	Mm
		476858 UBC_MOUSE (1-135): 9.67e-34: 126: 57.78		HUWE1_MOUSE (352-808): 7.81e-167: 538: 37.42
+	Hs
		475080 UBB_HUMAN (1-76): 4.1e-32: 115: 96.05		HUWE1_HUMAN (252-808): 3.56e-167: 539: 31.78
+	Sc
		477647 RL40A_YEAST (1-76): 1.13e-42: 139: 100.00		TOM1_YEAST (370-808): 5.87e-157: 509: 37.36
+	Sp
		143368 UBI4P_SCHPO (1-76): 1.14e-32: 120: 100.00		PTR1_SCHPO (396-807): 6.41e-159: 515: 39.67
+	Sc
		477424 UBI4P_YEAST (1-76): 1.31e-32: 120: 100.00		TOM1_YEAST (370-808): 5.87e-157: 509: 37.36
+	Dm
		89880 RL40_DROME (1-76): 8.42e-35: 119: 96.05		D5SHR3_DROME (397-809): 9.61e-173: 506: 46.19
+	Dm
		1376481 Q9W418_DROME (1-77): 2.23e-30: 116: 94.81		Q9W325_DROME (383-809): 1.16e-171: 503: 44.83