HitPredict

Interactor 1: TSAD (P05852)
tRNA N6-adenosine threonylcarbamoyltransferase (EC 2.3.1.234) (N6-L-threonylcarbamoyladenine synthase) (t(6)A synthase) (t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD) (tRNA threonylcarbamoyladenosine biosynthesis protein TsaD)
Gene: TSAD

Interactor 2: TSAB (P76256)
tRNA threonylcarbamoyladenosine biosynthesis protein TsaB (t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaB)
Gene: TSAB

Species: Escherichia coli K-12

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.68	1.00	0.826

Property	Details
Experiments	24561554, 15690043
Identification Methods	two hybrid, tandem affinity purification, pull down, two hybrid array
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	TsaD~TsaD
Common GO terms	GO:0000408 - EKC/KEOPS complex GO:0002949 - tRNA threonylcarbamoyladenosine modification GO:0005829 - cytosol GO:1990145 - maintenance of translational fidelity
Homologous interactions	7

Homologous interactions

Color coding for hit scores

	1 337 Ec			1 231
+	Ec
		409401 TSAD_ECOLI (1-337): 1.68e-165: 466: 100.00		TSAD_ECOLI (1-157): 1.35e-39: 141: 20.46
+	Hs
		1342726 OSGEP_HUMAN (3-328): 7.77e-144: 411: 34.64		OSGEP_HUMAN (3-160): 7.77e-56: 183: 21.51
+	Nv
		1175744 TSAD_NITV2 (1-328): 2.87e-133: 385: 42.36		Q72DR5_NITV2 (4-219): 6.39e-62: 196: 24.43
+	Ss
		937041 TSAD_SYNY3 (2-334): 3.51e-137: 394: 40.88		P72633_SYNY3 (4-217): 3.85e-47: 156: 27.43
+	Sc
		733424 KAE1_YEAST (2-328): 1.05e-122: 359: 27.00		KAE1_YEAST (2-160): 6.42e-42: 147: 12.04
+	Sc
		793554 QRI7_YEAST (2-334): 3.27e-125: 366: 33.06		QRI7_YEAST (2-158): 8.76e-34: 127: 18.79
+	Ec
		940175 TSAB_ECOLI (1-109): 1.35e-26: 107: 28.44		TSAB_ECOLI (1-231): 1.02e-97: 285: 100.00