HitPredict

Interactor 1: HS90B (P08238)
Heat shock protein HSP 90-beta (HSP 90) (Heat shock 84 kDa) (HSP 84) (HSP84) (Heat shock protein family C member 3)
Gene: HSP90AB1

Interactor 2: E2AK1 (Q9BQI3)
Eukaryotic translation initiation factor 2-alpha kinase 1 (EC 2.7.11.1) (Heme-controlled repressor) (HCR) (Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase) (Heme-regulated inhibitor) (hHRI) (Hemin-sensitive initiation factor 2-alpha kinase)
Gene: EIF2AK1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.62	1.00	0.789

Property	Details
Experiments	32707033, 22939624
Identification Methods	affinity chromatography technology, pull down, luminescence based mammalian interactome mapping
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	HSP90~Pkinase
Common GO terms	GO:0005524 - ATP binding GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0042803 - protein homodimerization activity
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 724 Hs			1 630
+	Hs
		438350 HS90B_HUMAN (1-724): 0: 1086: 100.00		DCLK2_HUMAN (114-603): 1.93e-110: 350: 15.45
+	Hs
		131545 HS90B_HUMAN (1-724): 0: 1086: 100.00		STK25_HUMAN (309-630): 3.82e-65: 221: 18.83
+	Hs
		438441 HS90B_HUMAN (1-724): 0: 1086: 100.00		KCC1G_HUMAN (294-583): 1.12e-62: 216: 22.85
+	Hs
		131544 HS90A_HUMAN (1-724): 0: 1072: 85.25		STK25_HUMAN (309-630): 3.82e-65: 221: 18.83
+	Hs
		437881 HS90B_HUMAN (1-724): 0: 1086: 100.00		E2AK2_HUMAN (380-580): 1.11e-21: 101: 34.16
+	Hs
		438633 HS90B_HUMAN (1-724): 0: 1086: 100.00		AKT3_HUMAN (126-313): 4.87e-21: 98.6: 17.99
+	Mm
		513660 HS90B_MOUSE (1-724): 0: 1089: 99.59		AKT1_MOUSE (127-313): 7.02e-19: 92: 17.46
+	Hs
		437928 HS90B_HUMAN (1-724): 0: 1086: 100.00		AKT2_HUMAN (122-318): 3.98e-19: 92.8: 14.85
+	Hs
		437927 HS90B_HUMAN (1-724): 0: 1086: 100.00		AKT1_HUMAN (127-313): 1.23e-18: 91.3: 16.93
+	Bt
		964601 HS90B_BOVIN (1-724): 0: 1085: 99.59		AKT1_BOVIN (127-313): 1.22e-18: 91.3: 16.93
+	Hs
		437870 HS90B_HUMAN (1-724): 0: 1086: 100.00		KAPCA_HUMAN (128-308): 1.68e-18: 89.3: 15.93
+	Hs
		431521 HS90A_HUMAN (1-724): 0: 1072: 85.25		E2AK2_HUMAN (380-580): 1.11e-21: 101: 34.16
+	Hs
		437900 HS90B_HUMAN (1-724): 0: 1086: 100.00		KAPCB_HUMAN (127-308): 6.67e-17: 84.3: 15.85
+	Hs
		431572 HS90A_HUMAN (1-724): 0: 1072: 85.25		AKT1_HUMAN (127-313): 1.23e-18: 91.3: 16.93
+	Hs
		438014 HS90B_HUMAN (1-724): 0: 1086: 100.00		NEK2_HUMAN (380-585): 5.13e-14: 76.6: 30.95
+	Hs
		431511 HS90A_HUMAN (1-724): 0: 1072: 85.25		KAPCA_HUMAN (128-308): 1.68e-18: 89.3: 15.93
+	Hs
		373549 HS90B_HUMAN (1-724): 0: 1086: 100.00		RAF1_HUMAN (422-586): 1.23e-12: 72.8: 31.25
+	Hs
		431538 HS90A_HUMAN (1-724): 0: 1072: 85.25		KAPCB_HUMAN (127-308): 6.67e-17: 84.3: 15.85
+	Hs
		70435 HS90A_HUMAN (1-724): 0: 1072: 85.25		B2RB89_HUMAN (127-308): 6.67e-17: 84.3: 15.85
+	Hs
		373543 HS90A_HUMAN (1-724): 0: 1072: 85.25		RAF1_HUMAN (422-586): 1.23e-12: 72.8: 31.25