HitPredict

Interactor 1: ISCS (P0A6B7)
Cysteine desulfurase IscS (EC 2.8.1.7) (NifS protein homolog) (ThiI transpersulfidase) (TusA transpersulfidase)
Gene: ISCS

Interactor 2: ISCU (P0ACD4)
Iron-sulfur cluster assembly scaffold protein IscU (Sulfur acceptor protein IscU)
Gene: ISCU

Species: Escherichia coli K-12

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.83	1.00	0.915

Property	Details
Experiments	16606699, 19305405, 15690043, 11972033
Identification Methods	affinity chromatography technology, enzymatic study, classical fluorescence spectroscopy, pull down, tandem affinity purification
Interaction Type	physical association, direct interaction, association, covalent binding, enzymatic reaction

Property	Details
Interacting Pfam domains	Aminotran_5~NifU_N
Common GO terms	GO:0005829 - cytosol GO:0016226 - iron-sulfur cluster assembly GO:0019448 - L-cysteine catabolic process GO:0051537 - 2 iron, 2 sulfur cluster binding GO:1990330 - IscS-IscU complex
Homologous interactions	7

Homologous interactions

Color coding for hit scores

	1 404 Ec			1 128
+	Hs
		1328771 NFS1_HUMAN (1-404): 1.01e-143: 418: 59.75		ISCU_HUMAN (3-128): 1.84e-66: 199: 75.40
+	Sc
		630685 NFS1_YEAST (3-404): 1.43e-139: 409: 56.47		ISU1_YEAST (3-126): 3.01e-65: 196: 72.00
+	Sc
		630691 NFS1_YEAST (3-404): 1.43e-139: 409: 56.47		ISU2_YEAST (3-127): 1.62e-64: 194: 73.02
+	Ec
		457421 ISCS_ECO57 (1-404): 2.4e-138: 402: 100.00		ISCU_ECO57 (1-128): 3.34e-66: 198: 100.00
+	Cj
		1011362 Q0PBR4_CAMJE (4-390): 1.21e-99: 303: 38.27		Q0PBR5_CAMJE (3-123): 1.08e-46: 154: 39.55
+	Nv
		1175890 Q72EB4_NITV2 (5-388): 7.98e-99: 301: 44.70		Q72EB3_NITV2 (3-124): 3.17e-44: 147: 49.59
+	Bs
		191733 SUFS_BACSU (5-384): 4.68e-96: 294: 22.17		SUFU_BACSU (3-123): 7.44e-37: 124: 29.29