HitPredict

Interactor 1: DNAK (P0A6Y8)
Chaperone protein DnaK (HSP70) (Heat shock 70 kDa protein) (Heat shock protein 70)
Gene: DNAK

Interactor 2: NIKD (P33593)
Nickel import ATP-binding protein NikD (EC 7.2.2.11)
Gene: NIKD

Species: Escherichia coli K-12

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.62	0.60	0.612

Property	Details
Experiments	16606699, 15690043
Identification Methods	affinity chromatography technology, pull down, tandem affinity purification
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005524 - ATP binding GO:0005886 - plasma membrane GO:0016020 - membrane GO:0016887 - ATP hydrolysis activity
Homologous interactions	5

Homologous interactions

Color coding for hit scores

	1 638 Ec			1 254
+	Ec
		459222 DNAK_ECOLI (1-638): 0: 871: 100.00		METN_ECOLI (4-251): 7.75e-76: 235: 27.91
+	Ec
		459167 DNAK_ECOLI (1-638): 0: 871: 100.00		GLNQ_ECOLI (4-242): 6.56e-76: 231: 23.36
+	Ec
		459251 DNAK_ECOLI (1-638): 0: 871: 100.00		DPPF_ECOLI (3-251): 4.56e-72: 225: 29.48
+	Ec
		459241 DNAK_ECOLI (1-638): 0: 871: 100.00		YEJF_ECOLI (18-243): 2.36e-68: 221: 34.47
+	Ec
		459239 DNAK_ECOLI (1-638): 0: 871: 100.00		NIKE_ECOLI (16-221): 2.29e-24: 99.8: 35.81