HitPredict

Interactor 1: EVGA (P0ACZ4)
DNA-binding transcriptional activator EvgA
Gene: EVGA

Interactor 2: NTRC (P0AFB8)
DNA-binding transcriptional regulator NtrC (Nitrogen regulation protein NR(I)) (Nitrogen regulator I) (NRI)
Gene: glnG

Species: Escherichia coli K-12

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.58	1.00	0.764

Property	Details
Experiments	24561554
Identification Methods	two hybrid, affinity chromatography technology, two hybrid array, pull down, luminescence based mammalian interactome mapping
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	GerE~Response_reg, Response_reg~Response_reg, Response_reg~Sigma54_activat
Common GO terms	GO:0005829 - cytosol GO:0006355 - regulation of DNA-templated transcription GO:0043565 - sequence-specific DNA binding
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 204 Ec			1 469
+	Mx
		1091425 Q1D1K9_MYXXD (2-202): 2.1e-49: 161: 16.51		Q1D0A2_MYXXD (2-468): 2.04e-152: 443: 36.03
+	Mx
		1091395 Q1CZP3_MYXXD (2-203): 1.36e-46: 154: 16.90		Q1D0A2_MYXXD (2-468): 2.04e-152: 443: 36.03
+	Mx
		1091406 Q1D8M8_MYXXD (3-200): 5.61e-47: 156: 16.74		Q1D033_MYXXD (5-468): 8.15e-151: 439: 42.55
+	Mx
		1091289 Q1D8M8_MYXXD (3-200): 5.61e-47: 156: 16.74		Q1CWG7_MYXXD (2-469): 8.91e-148: 432: 38.46
+	Ec
		467556 EVGA_ECOLI (1-204): 7.73e-47: 154: 100.00		FHLA_ECOLI (57-469): 1.18e-116: 359: 35.10
+	Ss
		937544 P73864_SYNY3 (1-203): 1.07e-48: 159: 18.98		P72948_SYNY3 (4-249): 7.84e-51: 174: 19.68
+	Ec
		468921 PHOB_ECOLI (2-202): 1.24e-52: 170: 14.09		PHOB_ECOLI (1-218): 3.3e-45: 159: 20.09
+	Ec
		465608 PHOB_ECOLI (2-202): 1.24e-52: 170: 14.09		OMPR_ECOLI (2-188): 8.98e-40: 145: 22.46
+	Mx
		1091446 Q1D8M8_MYXXD (3-200): 5.61e-47: 156: 16.74		Q1D1K9_MYXXD (5-216): 9.9e-45: 157: 26.61
+	Mx
		1091499 Q1D4T4_MYXXD (2-202): 6.44e-50: 163: 14.73		Q1D4T4_MYXXD (2-233): 4.41e-42: 150: 20.34
+	Ec
		935858 BAER_ECOLI (2-204): 1.04e-47: 158: 15.39		BAER_ECOLI (4-256): 3.75e-42: 151: 19.76
+	Ec
		940185 HPRR_ECOLI (1-202): 1.29e-47: 157: 17.05		HPRR_ECOLI (4-204): 6.7e-43: 152: 18.54
+	Bt
		1206284 Q89YR8_BACTN (2-203): 7.67e-45: 160: 16.75		Q89YR8_BACTN (2-164): 1.17e-37: 148: 28.49
+	Ec
		467557 EVGA_ECOLI (1-204): 7.73e-47: 154: 100.00		EVGS_ECOLI (2-200): 3.91e-39: 153: 18.50
+	Ec
		467567 HPRR_ECOLI (1-202): 1.29e-47: 157: 17.05		CUSR_ECOLI (4-216): 9.59e-42: 150: 17.73
+	Ec
		465606 OMPR_ECOLI (2-199): 1.24e-46: 155: 13.58		CPXR_ECOLI (5-237): 1.1e-41: 150: 20.25
+	Ec
		465604 OMPR_ECOLI (2-199): 1.24e-46: 155: 13.58		OMPR_ECOLI (2-188): 8.98e-40: 145: 22.46
+	Ec
		621089 PHOP_ECOLI (1-202): 1.02e-46: 154: 14.68		PHOP_ECOLI (5-181): 5.84e-39: 142: 22.03
+	Af
		1176516 Q7CXM1_AGRFC (2-204): 2.56e-46: 154: 16.06		Q7CXM1_AGRFC (4-167): 7.56e-38: 139: 26.83