HitPredict

Interactor 1: UBB (P0CG47)
Polyubiquitin-B [Cleaved into: Ubiquitin]
Gene: UBB

Interactor 2: DNJB2 (P25686)
DnaJ homolog subfamily B member 2 (Heat shock 40 kDa protein 3) (Heat shock protein J1) (HSJ-1)
Gene: DNAJB2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.74	0.60	0.667

Property	Details
Experiments	26186194, 28514442, 33961781
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005634 - nucleus GO:0005789 - endoplasmic reticulum membrane GO:0005829 - cytosol GO:0031398 - positive regulation of protein ubiquitination GO:0031625 - ubiquitin protein ligase binding
Homologous interactions	17

Homologous interactions

Color coding for hit scores

	1 229 Hs			1 324
+	Mm
		477042 UBC_MOUSE (1-181): 6.49e-62: 207: 96.13		DNJB2_MOUSE (1-324): 9.33e-127: 366: 85.19
+	Hs
		475356 UBC_HUMAN (1-153): 4.05e-53: 183: 99.35		DNJB2_HUMAN (1-324): 1.9e-125: 363: 100.00
+	Hs
		636473 RS27A_HUMAN (153-228): 1.76e-44: 147: 100.00		DNJB2_HUMAN (1-324): 1.9e-125: 363: 100.00
+	Rn
		88985 UBC_RAT (1-181): 2.6e-61: 206: 96.13		D4ABC2_RAT (1-274): 6.62e-93: 278: 82.48
+	Hs
		397783 ISG15_HUMAN (3-154): 1.46e-26: 102: 32.26		DNJB2_HUMAN (1-324): 1.9e-125: 363: 100.00
+	Mm
		222718 UBC_MOUSE (1-181): 6.49e-62: 207: 96.13		DNJB6_MOUSE (1-273): 4.6e-75: 236: 41.28
+	Hs
		257894 UBC_HUMAN (1-153): 4.05e-53: 183: 99.35		DNJB6_HUMAN (1-283): 7.47e-82: 252: 47.63
+	Mm
		93712 UBC_MOUSE (1-181): 6.49e-62: 207: 96.13		DJC21_MOUSE (1-317): 1.74e-68: 224: 22.12
+	Rn
		1140400 UBC_RAT (1-181): 2.6e-61: 206: 96.13		Q5XIP0_RAT (2-239): 7.23e-45: 157: 28.05
+	Hs
		257885 ISG15_HUMAN (3-154): 1.46e-26: 102: 32.26		DNJB6_HUMAN (1-283): 7.47e-82: 252: 47.63
+	Hs
		475355 UBC_HUMAN (1-153): 4.05e-53: 183: 99.35		DNJB1_HUMAN (2-161): 3.26e-43: 153: 40.63
+	At
		1012787 UBQ3_ARATH (1-153): 2.79e-55: 180: 95.43		Q0WN54_ARATH (3-238): 1.64e-42: 155: 25.10
+	At
		67521 UBQ3_ARATH (1-153): 2.79e-55: 180: 95.43		A8MR40_ARATH (2-105): 2.09e-42: 149: 50.45
+	Hs
		1076133 OASL_HUMAN (1-153): 3.31e-21: 93.2: 29.11		DJB11_HUMAN (2-228): 1.38e-51: 175: 27.31
+	Hs
		279269 ISG15_HUMAN (3-154): 1.46e-26: 102: 32.26		DNJB5_HUMAN (2-239): 5.52e-44: 155: 25.88
+	Hs
		398129 ISG15_HUMAN (3-154): 1.46e-26: 102: 32.26		DNJB4_HUMAN (2-238): 5.01e-44: 155: 28.98
+	Hs
		397782 ISG15_HUMAN (3-154): 1.46e-26: 102: 32.26		DNJB1_HUMAN (2-161): 3.26e-43: 153: 40.63