HitPredict

Interactor 1: UBB (P0CG47)
Polyubiquitin-B [Cleaved into: Ubiquitin]
Gene: UBB

Interactor 2: ITCH (Q96J02)
E3 ubiquitin-protein ligase Itchy homolog (Itch) (EC 2.3.2.26) (Atrophin-1-interacting protein 4) (AIP4) (HECT-type E3 ubiquitin transferase Itchy homolog) (NFE2-associated polypeptide 1) (NAPP1)
Gene: ITCH

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.41	1.00	0.647

Property	Details
Experiments	17719543
Identification Methods	enzymatic study
Interaction Type	enzymatic reaction

Property	Details
Interacting Pfam domains	HECT~ubiquitin
Common GO terms	GO:0005654 - nucleoplasm GO:0005739 - mitochondrion GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0016567 - protein ubiquitination and 1 others more... GO:0070062 - extracellular exosome
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 229 Hs			1 903
+	Hs
		476205 UBC_HUMAN (1-153): 4.05e-53: 183: 99.35		ITCH_HUMAN (1-903): 0: 926: 100.00
+	Hs
		125112 UBB_HUMAN (1-229): 4.07e-98: 286: 100.00		WWP2_HUMAN (1-903): 0: 816: 54.08
+	Rn
		1140970 UBC_RAT (1-181): 2.6e-61: 206: 96.13		Q5YB86_RAT (11-903): 0: 866: 90.73
+	Hs
		476350 UBC_HUMAN (1-153): 4.05e-53: 183: 99.35		WWP1_HUMAN (1-903): 0: 832: 57.54
+	Hs
		125113 UBC_HUMAN (1-153): 4.05e-53: 183: 99.35		WWP2_HUMAN (1-903): 0: 816: 54.08
+	Hs
		474953 UBB_HUMAN (1-229): 4.07e-98: 286: 100.00		NEDD4_HUMAN (90-900): 0: 673: 34.16
+	Hs
		476229 UBC_HUMAN (1-153): 4.05e-53: 183: 99.35		NED4L_HUMAN (5-900): 0: 762: 32.14
+	Hs
		475470 UBC_HUMAN (1-153): 4.05e-53: 183: 99.35		NEDD4_HUMAN (90-900): 0: 673: 34.16
+	Hs
		475080 UBB_HUMAN (1-229): 4.07e-98: 286: 100.00		HUWE1_HUMAN (415-902): 1.81e-160: 523: 34.88
+	Ce
		477557 UBIQ1_CAEEL (1-228): 9.95e-87: 275: 98.25		Q9GUP2_CAEEL (488-901): 1.03e-160: 523: 38.97
+	Sp
		143368 UBI4P_SCHPO (1-228): 2.98e-90: 272: 96.05		PTR1_SCHPO (486-901): 1.43e-157: 514: 38.92
+	Dm
		1376481 Q9W418_DROME (1-228): 2.1e-92: 282: 100.00		Q9W325_DROME (496-903): 1.26e-168: 499: 46.38
+	Sc
		477424 UBI4P_YEAST (1-228): 5.66e-90: 271: 96.05		TOM1_YEAST (487-902): 5.57e-156: 510: 39.10
+	Mm
		476858 UBC_MOUSE (1-181): 6.49e-62: 207: 96.13		HUWE1_MOUSE (415-902): 1.58e-160: 523: 34.88
+	Hs
		475984 UBC_HUMAN (1-153): 4.05e-53: 183: 99.35		HUWE1_HUMAN (415-902): 1.81e-160: 523: 34.88
+	Hs
		475196 UBB_HUMAN (1-229): 4.07e-98: 286: 100.00		SMUF2_HUMAN (1-358): 2.56e-46: 180: 16.94
+	Sc
		477293 UBI4P_YEAST (1-228): 5.66e-90: 271: 96.05		RSP5_YEAST (16-440): 9.75e-40: 160: 19.07
+	Hs
		476414 UBC_HUMAN (1-153): 4.05e-53: 183: 99.35		SMUF1_HUMAN (1-535): 1.85e-47: 183: 14.71
+	Hs
		476403 UBC_HUMAN (1-153): 4.05e-53: 183: 99.35		SMUF2_HUMAN (1-358): 2.56e-46: 180: 16.94
+	Hs
		475963 UBC_HUMAN (1-153): 4.05e-53: 183: 99.35		HECW1_HUMAN (26-479): 2.25e-13: 76.9: 10.76