HitPredict

Interactor 1: UBB (P0CG47)
Polyubiquitin-B [Cleaved into: Ubiquitin]
Gene: UBB

Interactor 2: SMUF2 (Q9HAU4)
E3 ubiquitin-protein ligase SMURF2 (hSMURF2) (EC 2.3.2.26) (HECT-type E3 ubiquitin transferase SMURF2) (SMAD ubiquitination regulatory factor 2) (SMAD-specific E3 ubiquitin-protein ligase 2)
Gene: SMURF2

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.60	1.00	0.778

Property	Details
Experiments	17719543, 14755250
Identification Methods	enzymatic study, anti tag coimmunoprecipitation
Interaction Type	enzymatic reaction, ubiquitination reaction

Property	Details
Interacting Pfam domains	HECT~ubiquitin
Common GO terms	GO:0005634 - nucleus GO:0005654 - nucleoplasm GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0016567 - protein ubiquitination
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 229 Hs			1 748
+	Hs
		476403 UBC_HUMAN (1-153): 4.05e-53: 183: 99.35		SMUF2_HUMAN (1-748): 0: 834: 100.00
+	Hs
		476414 UBC_HUMAN (1-153): 4.05e-53: 183: 99.35		SMUF1_HUMAN (1-748): 0: 748: 70.44
+	Hs
		474953 UBB_HUMAN (1-229): 4.07e-98: 286: 100.00		NEDD4_HUMAN (104-746): 0: 628: 38.73
+	Sc
		477293 UBI4P_YEAST (1-228): 5.66e-90: 271: 96.05		RSP5_YEAST (11-748): 0: 638: 34.33
+	Hs
		475080 UBB_HUMAN (1-229): 4.07e-98: 286: 100.00		HUWE1_HUMAN (114-747): 0: 589: 31.46
+	Rn
		1140970 UBC_RAT (1-181): 2.6e-61: 206: 96.13		Q5YB86_RAT (139-748): 0: 658: 40.86
+	Hs
		475470 UBC_HUMAN (1-153): 4.05e-53: 183: 99.35		NEDD4_HUMAN (104-746): 0: 628: 38.73
+	Sp
		143368 UBI4P_SCHPO (1-228): 2.98e-90: 272: 96.05		PTR1_SCHPO (317-746): 3.86e-162: 522: 39.40
+	Mm
		476858 UBC_MOUSE (1-181): 6.49e-62: 207: 96.13		HUWE1_MOUSE (152-747): 0: 584: 33.28
+	Ce
		477557 UBIQ1_CAEEL (1-228): 9.95e-87: 275: 98.25		Q9GUP2_CAEEL (145-746): 4.01e-159: 513: 30.49
+	Sc
		477424 UBI4P_YEAST (1-228): 5.66e-90: 271: 96.05		TOM1_YEAST (325-747): 1.36e-156: 505: 40.47
+	Hs
		475984 UBC_HUMAN (1-153): 4.05e-53: 183: 99.35		HUWE1_HUMAN (114-747): 0: 589: 31.46
+	Dm
		1376481 Q9W418_DROME (1-228): 2.1e-92: 282: 100.00		Q9W325_DROME (343-748): 8.33e-164: 481: 42.48
+	Hs
		125112 UBB_HUMAN (1-229): 4.07e-98: 286: 100.00		WWP2_HUMAN (1-416): 7.64e-26: 116: 17.39
+	Hs
		475140 UBB_HUMAN (1-229): 4.07e-98: 286: 100.00		ITCH_HUMAN (1-426): 2.45e-23: 107: 16.44
+	Hs
		476229 UBC_HUMAN (1-153): 4.05e-53: 183: 99.35		NED4L_HUMAN (5-396): 1.96e-36: 149: 18.58
+	Hs
		125113 UBC_HUMAN (1-153): 4.05e-53: 183: 99.35		WWP2_HUMAN (1-416): 7.64e-26: 116: 17.39
+	Hs
		476205 UBC_HUMAN (1-153): 4.05e-53: 183: 99.35		ITCH_HUMAN (1-426): 2.45e-23: 107: 16.44
+	Hs
		476350 UBC_HUMAN (1-153): 4.05e-53: 183: 99.35		WWP1_HUMAN (2-395): 4.38e-20: 97.5: 15.47
+	Hs
		475963 UBC_HUMAN (1-153): 4.05e-53: 183: 99.35		HECW1_HUMAN (19-213): 6.29e-17: 87.9: 15.94