HitPredict

Interactor 1: UBC (P0CG48)
Polyubiquitin-C [Cleaved into: Ubiquitin]
Gene: UBC

Interactor 2: HUWE1 (Q7Z6Z7)
E3 ubiquitin-protein ligase HUWE1 (EC 2.3.2.26) (ARF-binding protein 1) (ARF-BP1) (HECT, UBA and WWE domain-containing protein 1) (HECT-type E3 ubiquitin transferase HUWE1) (Homologous to E6AP carboxyl terminus homologous protein 9) (HectH9) (Large structure of UREB1) (LASU1) (Mcl-1 ubiquitin ligase E3) (Mule) (Upstream regulatory element-binding protein 1) (URE-B1) (URE-binding protein 1)
Gene: HUWE1

Species: Homo sapiens

Source: biogrid

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.92	1.00	0.964

Property	Details
Experiments	25147182, 25723849, 28943312, 28190767, 31699778, 38803224, 23314748, 17550899
Identification Methods	affinity chromatography technology, enzymatic study, pull down
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	HECT~ubiquitin, UBA~ubiquitin, UBM~ubiquitin
Common GO terms	GO:0003723 - RNA binding GO:0005634 - nucleus GO:0005654 - nucleoplasm GO:0005829 - cytosol GO:0070062 - extracellular exosome
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 685 Hs			1 4374
+	Mm
		924239 RS27A_MOUSE (609-684): 9.1e-41: 147: 100.00		HUWE1_MOUSE (1-4374): 0: 3203: 96.37
+	Mm
		476858 UBC_MOUSE (1-108): 9.43e-30: 127: 90.74		HUWE1_MOUSE (1-4374): 0: 3203: 96.37
+	Hs
		476205 UBC_HUMAN (1-685): 0: 846: 100.00		ITCH_HUMAN (3507-4372): 0: 672: 23.10
+	Hs
		125113 UBC_HUMAN (1-685): 0: 846: 100.00		WWP2_HUMAN (3506-4372): 0: 624: 24.57
+	Hs
		476414 UBC_HUMAN (1-685): 0: 846: 100.00		SMUF1_HUMAN (3772-4371): 0: 620: 32.84
+	Hs
		476229 UBC_HUMAN (1-685): 0: 846: 100.00		NED4L_HUMAN (3542-4372): 0: 618: 27.53
+	Hs
		476350 UBC_HUMAN (1-685): 0: 846: 100.00		WWP1_HUMAN (3507-4372): 0: 613: 22.55
+	Hs
		476403 UBC_HUMAN (1-685): 0: 846: 100.00		SMUF2_HUMAN (3771-4371): 0: 611: 33.44
+	Hs
		475470 UBC_HUMAN (1-685): 0: 846: 100.00		NEDD4_HUMAN (3104-4372): 2.46e-179: 594: 21.46
+	Hs
		475963 UBC_HUMAN (1-685): 0: 846: 100.00		HECW1_HUMAN (3082-4373): 1.49e-161: 549: 18.31
+	Dm
		89879 UBIQP_DROME (1-610): 0: 752: 99.84		D5SHR3_DROME (3965-4373): 1.32e-179: 561: 37.14
+	Dm
		1376481 Q9W418_DROME (229-685): 0: 557: 99.78		Q9W325_DROME (3947-4373): 8.19e-180: 562: 35.58
+	Sp
		143368 UBI4P_SCHPO (305-685): 1.76e-151: 445: 95.80		PTR1_SCHPO (3681-4374): 2.02e-167: 587: 35.54
+	Sc
		477293 UBI4P_YEAST (381-685): 5e-122: 370: 95.74		RSP5_YEAST (3659-4372): 0: 595: 27.62
+	Sc
		477424 UBI4P_YEAST (381-685): 5e-122: 370: 95.74		TOM1_YEAST (3831-4374): 1.99e-157: 554: 43.77
+	Ce
		477557 UBIQ1_CAEEL (1-229): 5.97e-87: 292: 98.25		Q9GUP2_CAEEL (3471-4374): 1.65e-163: 575: 41.47
+	Hs
		125347 RS27A_HUMAN (609-684): 7.23e-41: 148: 100.00		WWP2_HUMAN (3506-4372): 0: 624: 24.57
+	Hs
		924156 RS27A_HUMAN (609-684): 7.23e-41: 148: 100.00		WWP1_HUMAN (3507-4372): 0: 613: 22.55
+	At
		1091758 UBQ3_ARATH (457-685): 3.23e-85: 272: 95.63		UPL1_ARATH (1343-3403): 5.18e-110: 398: 16.85
+	At
		1091762 UBQ3_ARATH (457-685): 3.23e-85: 272: 95.63		UPL2_ARATH (1671-2913): 1.97e-94: 347: 14.79