HitPredict

Interactor 1: UBC (P0CG48)
Polyubiquitin-C [Cleaved into: Ubiquitin]
Gene: UBC

Interactor 2: AMFR (Q9UKV5)
E3 ubiquitin-protein ligase AMFR (EC 2.3.2.36) (Autocrine motility factor receptor) (AMF receptor) (RING finger protein 45) (gp78)
Gene: AMFR

Species: Homo sapiens

Source: biogrid

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.96	1.00	0.980

Property	Details
Experiments	11724934, 31699778, 38803224, 23246001, 25409783, 15331598, 20126661, 21343306, 12847084, 23123110, 18216283, 16868077
Identification Methods	affinity chromatography technology, pull down, enzymatic study, x-ray crystallography
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	CUE~ubiquitin, ubiquitin~zf-RING_2
Common GO terms	GO:0005789 - endoplasmic reticulum membrane GO:0005829 - cytosol GO:0005886 - plasma membrane
Homologous interactions	18

Homologous interactions

Color coding for hit scores

	1 685 Hs			1 643
+	Hs
		476224 UBC_HUMAN (1-685): 0: 846: 100.00		RN145_HUMAN (72-428): 1.7e-63: 224: 17.25
+	Hs
		475339 UBC_HUMAN (1-685): 0: 846: 100.00		CBL_HUMAN (293-550): 4.72e-35: 143: 19.37
+	Hs
		476254 UBC_HUMAN (1-685): 0: 846: 100.00		RING2_HUMAN (315-579): 7.78e-32: 128: 14.44
+	Hs
		476628 UBC_HUMAN (1-685): 0: 846: 100.00		RN115_HUMAN (316-401): 6.66e-25: 107: 26.67
+	Hs
		475842 UBC_HUMAN (1-685): 0: 846: 100.00		RN151_HUMAN (336-382): 6.08e-10: 62.1: 31.92
+	Hs
		924212 RS27A_HUMAN (609-684): 7.23e-41: 148: 100.00		AMFR_HUMAN (1-643): 0: 593: 100.00
+	Mm
		477046 UBC_MOUSE (1-108): 9.43e-30: 127: 90.74		AMFR_MOUSE (1-643): 0: 604: 94.40
+	Sc
		477469 UBI4P_YEAST (381-685): 5e-122: 370: 95.74		HRD1_YEAST (73-500): 2.2e-47: 177: 14.54
+	Mm
		476671 UBB_MOUSE (457-685): 2.3e-86: 275: 99.56		RO52_MOUSE (337-629): 2.11e-32: 132: 14.97
+	At
		1091813 UBQ3_ARATH (457-685): 3.23e-85: 272: 95.63		RNG1L_ARATH (289-428): 2.87e-24: 106: 20.69
+	Rn
		1140402 UBC_RAT (1-108): 1.27e-29: 127: 90.74		MGRN1_RAT (303-626): 9.5e-49: 180: 16.92
+	Hs
		610336 RS27A_HUMAN (609-684): 7.23e-41: 148: 100.00		CBL_HUMAN (293-550): 4.72e-35: 143: 19.37
+	Mm
		924236 RS27A_MOUSE (609-684): 9.1e-41: 147: 100.00		RO52_MOUSE (337-629): 2.11e-32: 132: 14.97
+	Rn
		88778 UBC_RAT (1-108): 1.27e-29: 127: 90.74		D3ZV15_RAT (293-550): 7.57e-37: 149: 19.55
+	Mm
		476831 UBC_MOUSE (1-108): 9.43e-30: 127: 90.74		RO52_MOUSE (337-629): 2.11e-32: 132: 14.97
+	Mm
		476994 UBC_MOUSE (1-108): 9.43e-30: 127: 90.74		RN115_MOUSE (316-401): 6.53e-25: 107: 28.89
+	Hs
		398150 ISG15_HUMAN (228-380): 1.72e-28: 113: 32.69		RN115_HUMAN (316-401): 6.66e-25: 107: 26.67
+	Mm
		476947 UBC_MOUSE (1-108): 9.43e-30: 127: 90.74		RN126_MOUSE (318-389): 3.43e-10: 63.7: 32.90