HitPredict - High confidence protein-protein interactions

Interactor 1: R1AB (P0DTD1)
Replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) [Cleaved into: Host translation inhibitor nsp1 (Leader protein) (Non-structural protein 1) (nsp1); Non-structural protein 2 (nsp2) (p65 homolog); Papain-like protease nsp3 (EC 3.4.19.12) (EC 3.4.22.-) (Non-structural protein 3) (nsp3) (PL2-PRO) (Papain-like proteinase) (PL-PRO); Non-structural protein 4 (nsp4); 3C-like proteinase nsp5 (3CL-PRO) (3CLp) (EC 3.4.22.69) (Main protease) (Mpro) (Non-structural protein 5) (nsp5) (SARS coronavirus main proteinase); Non-structural protein 6 (nsp6); Non-structural protein 7 (nsp7); Non-structural protein 8 (nsp8); Viral protein genome-linked nsp9 (Non-structural protein 9) (nsp9) (RNA-capping enzyme subunit nsp9); Non-structural protein 10 (nsp10) (Growth factor-like peptide) (GFL); RNA-directed RNA polymerase nsp12 (Pol) (RdRp) (EC 2.7.7.48) (EC 2.7.7.50) (Non-structural protein 12) (nsp12); Helicase nsp13 (Hel) (EC 3.6.4.12) (EC 3.6.4.13) (Non-structural protein 13) (nsp13); Guanine-N7 methyltransferase nsp14 (EC 2.1.1.56) (EC 3.1.13.-) (Non-structural protein 14) (nsp14) (Proofreading exoribonuclease nsp14) (ExoN); Uridylate-specific endoribonuclease nsp15 (EC 4.6.1.-) (NendoU) (Non-structural protein 15) (nsp15); 2'-O-methyltransferase nsp16 (EC 2.1.1.57) (Non-structural protein 16) (nsp16)]
Gene: rep

Interactor 2: R1AB (P0DTD1)
Replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) [Cleaved into: Host translation inhibitor nsp1 (Leader protein) (Non-structural protein 1) (nsp1); Non-structural protein 2 (nsp2) (p65 homolog); Papain-like protease nsp3 (EC 3.4.19.12) (EC 3.4.22.-) (Non-structural protein 3) (nsp3) (PL2-PRO) (Papain-like proteinase) (PL-PRO); Non-structural protein 4 (nsp4); 3C-like proteinase nsp5 (3CL-PRO) (3CLp) (EC 3.4.22.69) (Main protease) (Mpro) (Non-structural protein 5) (nsp5) (SARS coronavirus main proteinase); Non-structural protein 6 (nsp6); Non-structural protein 7 (nsp7); Non-structural protein 8 (nsp8); Viral protein genome-linked nsp9 (Non-structural protein 9) (nsp9) (RNA-capping enzyme subunit nsp9); Non-structural protein 10 (nsp10) (Growth factor-like peptide) (GFL); RNA-directed RNA polymerase nsp12 (Pol) (RdRp) (EC 2.7.7.48) (EC 2.7.7.50) (Non-structural protein 12) (nsp12); Helicase nsp13 (Hel) (EC 3.6.4.12) (EC 3.6.4.13) (Non-structural protein 13) (nsp13); Guanine-N7 methyltransferase nsp14 (EC 2.1.1.56) (EC 3.1.13.-) (Non-structural protein 14) (nsp14) (Proofreading exoribonuclease nsp14) (ExoN); Uridylate-specific endoribonuclease nsp15 (EC 4.6.1.-) (NendoU) (Non-structural protein 15) (nsp15); 2'-O-methyltransferase nsp16 (EC 2.1.1.57) (Non-structural protein 16) (nsp16)]
Gene: rep

Species: Severe acute respiratory syndrome coronavirus 2

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.99	1.00	0.999

Property	Details
Experiments	32198291, 32272481, 888800000009, 888800000011, 32382072, 32531208, 888800000022, 32709886, 32277040, 32838362, 32855413, 888800000032, 888800000040, 33845483, 35773339, 33028810, 888800000054, 888800000072, 32841477, 33158912 and 181 others more... 888800000095, 33972410, 888800000137, 32783916, 33811162, 34174328, 888800000166, 33886614, 888800000183, 33705595, 33874769, 34131072, 888800000212, 34192965, 888800000221, 34031399, 33883267, 34341772, 35165203, 35021060, 888800000246, 34198327, 34198326, 34399606, 34198323, 34793155, 36071812, 35287429, 888800000293, 34437808, 34514483, 32304108, 33631104, 888800000372, 35432913, 34709727, 35389231, 888800000489, 888800000528, 35292745, 35801916, 888800000610, 36229406, 36490335, 888800000719, 36326185, 888800000767, 32709887, 888800000784, 888800000780, 888800000821, 34672954, 36217029, 33999154, 34769210, 35730969, 36095012, 34331944, 34078893, 34942146, 33956156, 34143953, 34847508, 34294141, 888800000877, 888800000894, 888800000897, 34781739, 888800000912, 888800000931, 888800000940, 33504779, 33503819, 33594727, 34197805, 35234150, 36057636, 35110538, 36040262, 35563658, 36773692, 35635946, 36179320, 34161756, 36334779, 37080108, 36941262, 37115000, 34315827, 35809383, 35807537, 888800000946, 32994211, 35709506, 36114420, 36920943, 34804549, 37047038, 35477935, 35944563, 35536318, 888800000994, 35600064, 37043904, 36335936, 36421689, 37044215, 37704626, 888800001008, 33159807, 33510133, 33036230, 32895623, 33795671, 888800001026, 35815070, 34270554, 33755450, 34083449, 34131140, 37943932, 36146880, 35779291, 34210738, 36775130, 36853058, 36575184, 33283984, 34242027, 33564093, 36796300, 888800001047, 888800001051, 888800001056, 38289714, 888800001065, 34528437, 34041264, 34347470, 35839690, 34506130, 35461811, 32747425, 37833261, 33152262, 34403466, 36725929, 32728018, 34426525, 34414360, 34213885, 33602867, 32321856, 888800001085, 36336176, 38499483, 34097796, 33054210, 33658206, 888800001099, 888800001101, 32541865, 888800001111, 32737471, 34278402, 888800001120, 888800001121, 32887884, 35128408, 35194144, unassigned2353, 32592996, 32745548, 32880863, 33024972, 33208735, unassigned2933, 34369011, 34189273, 34285133, 34542936
Identification Methods	x-ray crystallography, pull down, biochemical, two hybrid, affinity chromatography technology, proximity labelling technology, enzymatic study, fluorescent resonance energy transfer, protein complementation assay, molecular sieving and 17 others more... cosedimentation in solution, differential scanning calorimetry, dynamic light scattering, detection by mass spectrometry, mass spectrometry studies of complexes, x ray scattering, surface plasmon resonance, barcode fusion genetics two hybrid, validated two hybrid, split luciferase complementation, cross-linking study, small angle neutron scattering, protease assay, probe interaction assay, anti tag coimmunoprecipitation, uridylation assay, ion exchange chromatography
Interaction Type	association, direct interaction, physical association, cleavage reaction, covalent binding, enzymatic reaction, uridylation reaction

Property	Details
Interacting Pfam domains	AAA_12~AAA_12, bCoV_NAB~bCoV_NAB, Macro~bCoV_NSP3_N, CoV_ExoN~CoV_ExoN, CoV_ExoN~CoV_NSP10, CoV_ExoN~CoV_NSP9, CoV_ExoN~CoV_RPol_N, CoV_Methyltr_2~CoV_Methyltr_2, CoV_Methyltr_2~CoV_NSP10, CoV_NSP10~CoV_NSP10, CoV_NSP15_C~CoV_NSP15_C, CoV_NSP15_C~CoV_NSP15_M, CoV_NSP15_C~CoV_NSP15_N, CoV_NSP15_M~CoV_NSP15_M, CoV_NSP15_M~CoV_NSP15_N, CoV_NSP15_N~CoV_NSP15_N, CoV_NSP2_N~CoV_NSP2_N, CoV_NSP3_C~CoV_NSP3_C, CoV_NSP4_C~CoV_NSP4_C, CoV_NSP7~CoV_NSP7 and 14 others more... CoV_NSP7~CoV_NSP8, CoV_NSP7~RdRP_1, CoV_NSP8~CoV_NSP8, CoV_NSP8~CoV_RPol_N, CoV_NSP8~RdRP_1, CoV_NSP9~CoV_NSP9, CoV_NSP9~CoV_RPol_N, CoV_NSP9~RdRP_1, CoV_peptidase~CoV_peptidase, CoV_RPol_N~CoV_RPol_N, CoV_RPol_N~RdRP_1, Macro~Macro, Peptidase_C30~Peptidase_C30, RdRP_1~RdRP_1
Common GO terms	GO:0000175 - 3'-5'-RNA exonuclease activity GO:0002151 - G-quadruplex RNA binding GO:0003723 - RNA binding GO:0003727 - single-stranded RNA binding GO:0003968 - RNA-dependent RNA polymerase activity and 38 others more... GO:0004197 - cysteine-type endopeptidase activity GO:0004482 - mRNA 5'-cap (guanine-N7-)-methyltransferase activity GO:0004483 - mRNA (nucleoside-2'-O-)-methyltransferase activity GO:0004484 - mRNA guanylyltransferase activity GO:0004843 - cysteine-type deubiquitinase activity GO:0005507 - copper ion binding GO:0005524 - ATP binding GO:0006351 - DNA-templated transcription GO:0006508 - proteolysis GO:0008242 - omega peptidase activity GO:0008270 - zinc ion binding GO:0008289 - lipid binding GO:0016020 - membrane GO:0016829 - lyase activity GO:0016887 - ATP hydrolysis activity GO:0016892 - RNA endonuclease activity, producing 3'-phosphomonoesters GO:0019049 - virus-mediated perturbation of host defense response GO:0019082 - viral protein processing GO:0019785 - ISG15-specific peptidase activity GO:0032574 - 5'-3' RNA helicase activity GO:0039502 - symbiont-mediated suppression of host type I interferon-mediated signaling pathway GO:0039520 - induction by virus of host autophagy GO:0039548 - symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity GO:0039579 - symbiont-mediated suppression of host ISG15-protein conjugation GO:0039595 - symbiont-mediated degradation of host mRNA GO:0039648 - symbiont-mediated perturbation of host ubiquitin-like protein modification GO:0039694 - viral RNA genome replication GO:0039722 - symbiont-mediated suppression of host toll-like receptor signaling pathway GO:0039723 - symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of TBK1 activity GO:0042025 - host cell nucleus GO:0043139 - 5'-3' DNA helicase activity GO:0044167 - host cell endoplasmic reticulum membrane GO:0044172 - host cell endoplasmic reticulum-Golgi intermediate compartment GO:0044174 - host cell endosome GO:0044177 - host cell Golgi apparatus GO:0044220 - host cell perinuclear region of cytoplasm GO:0044600 - protein guanylyltransferase activity GO:0062243 - double membrane vesicle viral factory outer membrane
Homologous interactions	5

Homologous interactions

Color coding for hit scores

	1	7096
Sa

1	7096

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Last updated: 15 Jul 2024