HitPredict

Interactor 1: ARAF (P10398)
Serine/threonine-protein kinase A-Raf (EC 2.7.11.1) (Proto-oncogene A-Raf) (Proto-oncogene A-Raf-1) (Proto-oncogene Pks)
Gene: ARAF

Interactor 2: 1433Z (P63104)
14-3-3 protein zeta/delta (Protein kinase C inhibitor protein 1) (KCIP-1)
Gene: YWHAZ

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.95	1.00	0.979

Property	Details
Experiments	25266655, 35271311, 36931259, 25852190, 28205554, 29777862, 31980649, 32707033, 20936779, 19933840, unassigned2173, 20052757
Identification Methods	affinity chromatography technology, fluorescent resonance energy transfer, two hybrid, anti tag coimmunoprecipitation, peptide array, pull down, two hybrid pooling approach, coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	14-3-3~C1_1, 14-3-3~PK_Tyr_Ser-Thr
Common GO terms	GO:0005829 - cytosol GO:0043066 - negative regulation of apoptotic process
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 606 Hs			1 245
+	Hs
		488645 ARAF_HUMAN (1-606): 0: 579: 100.00		1433E_HUMAN (2-241): 9.9e-144: 403: 68.18
+	Hs
		488577 ARAF_HUMAN (1-606): 0: 579: 100.00		1433T_HUMAN (1-245): 2.6e-140: 395: 79.59
+	Hs
		488590 ARAF_HUMAN (1-606): 0: 579: 100.00		1433B_HUMAN (1-242): 9.01e-140: 393: 87.60
+	Hs
		110870 H7C560_HUMAN (3-604): 0: 547: 56.68		1433Z_HUMAN (1-245): 1.67e-144: 405: 100.00
+	Hs
		542857 BRAF_HUMAN (3-604): 0: 546: 56.68		1433Z_HUMAN (1-245): 1.67e-144: 405: 100.00
+	Hs
		110869 H7C560_HUMAN (3-604): 0: 547: 56.68		1433E_HUMAN (2-241): 9.9e-144: 403: 68.18
+	Hs
		542849 BRAF_HUMAN (3-604): 0: 546: 56.68		1433E_HUMAN (2-241): 9.9e-144: 403: 68.18
+	Gg
		982836 BRAF_CHICK (3-604): 0: 542: 56.20		1433Z_CHICK (1-245): 7.65e-144: 403: 99.18
+	Hs
		542820 BRAF_HUMAN (3-604): 0: 546: 56.68		1433T_HUMAN (1-245): 2.6e-140: 395: 79.59
+	Hs
		542827 BRAF_HUMAN (3-604): 0: 546: 56.68		1433B_HUMAN (1-242): 9.01e-140: 393: 87.60
+	Gg
		982840 BRAF_CHICK (3-604): 0: 542: 56.20		1433T_CHICK (1-245): 5.31e-140: 394: 79.59
+	Gg
		982838 BRAF_CHICK (3-604): 0: 542: 56.20		1433B_CHICK (1-242): 1.22e-129: 367: 87.60
+	Hs
		488639 ARAF_HUMAN (1-606): 0: 579: 100.00		1433G_HUMAN (1-240): 2.37e-108: 314: 74.59
+	Hs
		488691 ARAF_HUMAN (1-606): 0: 579: 100.00		1433F_HUMAN (2-240): 1.26e-107: 312: 74.49
+	Hs
		488591 ARAF_HUMAN (1-606): 0: 579: 100.00		1433S_HUMAN (1-237): 8.27e-104: 302: 71.97
+	Hs
		542847 BRAF_HUMAN (3-604): 0: 546: 56.68		1433G_HUMAN (1-240): 2.37e-108: 314: 74.59
+	Hs
		542868 BRAF_HUMAN (3-604): 0: 546: 56.68		1433F_HUMAN (2-240): 1.26e-107: 312: 74.49
+	Gg
		982833 BRAF_CHICK (3-604): 0: 542: 56.20		1433G_CHICK (1-240): 7.95e-109: 315: 74.59
+	Gg
		982837 BRAF_CHICK (3-604): 0: 542: 56.20		Q5ZKJ2_CHICK (2-240): 7.65e-108: 312: 74.49
+	Hs
		542828 BRAF_HUMAN (3-604): 0: 546: 56.68		1433S_HUMAN (1-237): 8.27e-104: 302: 71.97